Frequency-domain fluorescence spectroscopy

JR Lakowicz, I Gryczynski - Topics in Fluorescence Spectroscopy …, 1991 - Springer
… In the time domain one measures the time-… domain data provide excellent resolution of
time-dependent spectral parameters. It is possible to resolve closely spaced fluorescence lifetimes

Ruby crystal for demonstrating time-and frequency-domain methods of fluorescence lifetime measurements

DE Chandler, ZK Majumdar, GJ Heiss… - … of Fluorescence, 2006 - Springer
… measuring fluorescence lifetimes with both time- and frequency-domain methods. The sample
is ruby crystal, which has a lifetime … In the time domain this is an exponential decay. τ is the …

[HTML][HTML] Fluorescence lifetime heterogeneity resolution in the frequency domain by lifetime moments analysis

A Esposito, HC Gerritsen, FS Wouters - Biophysical journal, 2005 - cell.com
… method with lifetime detection in the time domain (TD) is presented. Here, fitting the time-resolved
fluorescence decays gives quantitative information about the lifetime heterogeneities. …

Polar plot representation for frequency-domain analysis of fluorescence lifetimes

GI Redford, RM Clegg - Journal of fluorescence, 2005 - Springer
fluorescence lifetime data acquired in the frequency domain. … There are many ways to acquire
data in the time domain, and … decay directly in the time domain requires that the repetition …

Selective plane illumination microscopy (SPIM) with time-domain fluorescence lifetime imaging microscopy (FLIM) for volumetric measurement of cleared mouse brain …

T Funane, SS Hou, KM Zoltowska… - Review of Scientific …, 2018 - pubs.aip.org
… with time-domain fluorescence lifetime imaging microscopy (SPIM-FLIM) system consists
of frequency-doubled laser-generation, light-sheet illumination, and time-domain FLIM …

Histogram clustering for rapid time-domain fluorescence lifetime image analysis

Y Li, N Sapermsap, J Yu, J Tian, Y Chen… - Biomedical Optics …, 2021 - opg.optica.org
… We propose a histogram clustering (HC) method to accelerate fluorescence lifetime imaging
(FLIM… (up to 106 times) but also enhances lifetime estimation accuracy. Fast lifetime analysis …

[PDF][PDF] High frame rate fluorescence lifetime imaging

AV Agronskaia, L Tertoolen… - Journal of Physics D …, 2003 - researchgate.net
… Typical times required for the acquisition of fluorescence lifetime images are on the order of
… to acquire fluorescence lifetime images: frequency domain, and timedomain based methods. …

A method for the fast and photon‐efficient analysis of timedomain fluorescence lifetime image data over large dynamic ranges

RF Laine, C Poudel, CF Kaminski - Journal of Microscopy, 2022 - Wiley Online Library
… Our approach extends the dynamic range of fluorescence lifetimes that can be … all time-domain
FLIM data sets and could also be implemented on-chip to achieve fast and real-time FLIM …

Time-resolved fluorescence imaging in biology and medicine

R Cubeddu, D Comelli, C D'Andrea… - Journal of Physics D …, 2002 - iopscience.iop.org
… In the time domain approach the fluorescence lifetime map of a sample is measured using …
that acquires only the fluorescence emission falling within a definite time interval. The detector …

Fluorescence lifetime imaging

JR Lakowicz, H Szmacinski, K Nowaczyk… - Analytical …, 1992 - Elsevier
… the sensitivity of fluorescence decay times to chemical and … fluorescence lifetimes with 2D
imaging, to create images in which the lifetimes are determined at each pixel and the lifetimes