Fluorescence lifetime imaging

JR Lakowicz, H Szmacinski, K Nowaczyk… - Analytical …, 1992 - Elsevier
… the sensitivity of fluorescence decay times to chemical and … fluorescence lifetimes with 2D
imaging, to create images in which the lifetimes are determined at each pixel and the lifetimes

Fluorescence Lifetime Spectroscopy of Glioblastoma Multiforme

L Marcu, JA Jo, PV Butte, WH Yong… - Photochemistry and …, 2004 - Wiley Online Library
… bands) and the time domain (average lifetime) measured at 390 … are characterized by
fluorescence lifetimes that varied with … Our study demonstrates that the use of fluorescence lifetime

Direct reconstruction method for time-domain fluorescence molecular lifetime tomography

C Cai, L Zhang, J Zhang, J Bai, J Luo - Optics Letters, 2015 - opg.optica.org
… accuracy for fluorescent targets, high quantification accuracy for fluorescence lifetime, and
Fluorescence lifetime is generally sensitive to the environment and is influenced by such …

Fluorescence-lifetime imaging techniques for microscopy

CY Dong, T French, PTC So, C Buehler… - Methods in cell …, 2003 - Elsevier
… : the time-domain or the frequency-domain approach (for further discussion, see Chapter
9). In the time domain, the impulse response of a system is probed. A fluorescent sample is …

Fluorescence lifetime filtering

LB McGown - Analytical Chemistry, 1989 - ACS Publications
… Longpass filters in the time domain are ideal for certain applications in which the desired
signal is much longer-lived than the background signal. For example, scattered light interfer…

Fluorescence lifetime measurements and biological imaging

MY Berezin, S Achilefu - Chemical reviews, 2010 - ACS Publications
… Frequency domain data obtained from conversion of time domain data shown in Figure 7
via Fourier transform. Left: Phase and modulation at 80 MHz frequency, excitation (black), …

Early-photon guided reconstruction method for time-domain fluorescence lifetime tomography

L Zhang, C Cai, Y Lv, J Luo - Chinese Optics Letters, 2016 - opg.optica.org
… , frequency-domain mode FMT, or time-domain (TD) mode FMT, while fluorescence lifetime
can … For specific kinds of fluorochromes, fluorescence lifetime is environmentally sensitive. In …

Fluorescence lifetime imaging by using time-gated data acquisition

VY Soloviev, KB Tahir, J McGinty, DS Elson… - Applied Optics, 2007 - opg.optica.org
… The inverse problem formulated as the reconstruction of the quantum yield and the lifetime
in the time domain on the basis of a system of equations of Eq. (25) type is almost intractable …

Fluorescence lifetime-based optical molecular imaging

ATN Kumar - Molecular Imaging: Methods and Protocols, 2011 - Springer
… exploit lifetime contrast using a time domain fluorescence … The time domain data are
optimally analyzed using a lifetime-… of lifetimes and decay amplitudes from the long time decay …

Analog mean-delay method for high-speed fluorescence lifetime measurement

S Moon, Y Won, DY Kim - Optics express, 2009 - opg.optica.org
… In our lifetime measurement method, the time-domain intensity signal of a fluorescence decay
… In our method, an analog fluorescence intensity signal is acquired in the time domain and …