… Development of the antigen retrieval (AR) technique, a simple method of boiling archival
paraffin-embedded tissue sections in water to enhance the signal of immunohistochemistry (IHC…

… without any antigen retrieval (AR), or following section digestion with 0·… method of antigen
retrieval for routinely processed tissues. J … Possible role of tissue-bound calcium ions in citrate-…

… sections without damaging the sections. We describe here a simple, reliable and sensitive
antigen retrieval … to yield effective antigen retrieval with minimal tissue damage in free-floating …

… One simple protocol of boiling FFPE tissue sections in a retrieval solution of Tris-HCl buffer
(pH 7 or 9) containing 2% SDS was developed in our laboratory, and the quality of protein …

… buffer (pH 3.0) was an excellent buffer for antigen retrieval but destroyed tissue structures. …
pattern of any of the antigens examined in our studies in either paraffin sections or frozen …

… A simple method of either heating the routinely fixed paraffin-embedded tissue sections
in water or immersing the routinely formalin-fixed, acid-decalcified, celloidin-embedded …

… tissue sections were subjected to the AR method, obtained a similar intensity of immunostaining
compared to a protocol widely used in immunohistochemical studies [15]. In the last …

… After sectioning, antigen retrieval … tissue during antigen retrieval techniques. We routinely
coat microscope slides with APES to ensure maximum section adhesion and minimum section …

… be some tissue component that exists in the same tissue section as the target antigen when
… to identify a quantifiable internal control in FFPE tissue sections for the following reasons: (1…

… In this study we present the results of a wide range of antibodies with respect to their … using
serial sections. In contrast to other published reports, we investigated not only tissues fixed …