The TOL network of Pseudomonas putida mt2 processes multiple environmental inputs into a narrow response space

R Silva‐Rocha, V de Lorenzo - Environmental microbiology, 2013 - Wiley Online Library
… of network attractors (Bornholdt, 2008). This analysis allowed us to identify and assess the
TOL … We discovered that the inner logic structure of the TOL network restricts the expression of …

Increasing Signal Specificity of the TOL Network of Pseudomonas putida mt-2 by Rewiring the Connectivity of the Master Regulator XylR

A de Las Heras, S Fraile, V de Lorenzo - 2012 - journals.plos.org
… operons encoded in the TOL plasmid pWW0 of Pseudomonas putida, … TOL operon, Pu),
the reporter system displayed an increased transcriptional capacity in the resulting network for …

Decoding the genetic networks of environmental bacteria: regulatory moonlighting of the TOL system of Pseudomonas putida mt-2

R Silva-Rocha, D Pérez-Pantoja… - The ISME journal, 2013 - academic.oup.com
… The more the TOL system is examined, the more traces we find that P. putida mt-2 has been
… the extant genetic and biochemical network has found solutions to (perhaps) all of them. …

The regulatory logic of m‐xylene biodegradation by Pseudomonas putida mt2 exposed by dynamic modelling of the principal node Ps/Pr of the TOL plasmid

M Koutinas, MC Lam, A Kiparissides… - Environmental …, 2010 - Wiley Online Library
… transcriptional control network of the TOL plasmid pWW0 born by Pseudomonas putida
mt-2 for … In order to penetrate the underlying logic of such a network, which controls a major …

High‐resolution analysis of the m‐xylene/toluene biodegradation subtranscriptome of Pseudomonas putida mt2

J Kim, D Pérez‐Pantoja, R Silva‐Rocha… - Environmental …, 2016 - Wiley Online Library
… Response of the TOL network to gratuitous induction and to 3MBz To complete the picture
of the response of the TOL network to its canonical substrate, m-xylene, we examined the …

Subcellular Architecture of the xyl Gene Expression Flow of the TOL Catabolic Plasmid of Pseudomonas putida mt-2

J Kim, A Goñi-Moreno, V de Lorenzo - Mbio, 2021 - Am Soc Microbiol
… FIG 1 Interplay between metabolic and regulatory components of the TOL network and …
mRNAs of the TOL operons of plasmid pWW0. For this experiment, P. putida mt-2 strain was …

Inferring the genetic network of m‐xylene metabolism through expression profiling of the xyl genes of Pseudomonas putida mt2

F Velázquez, V Parro, V de Lorenzo - Molecular microbiology, 2005 - Wiley Online Library
… To study the degree of synchronization of the onsets of both the upper and the lower TOL
pathways, we measured the response of P. putida mt-2 to subsaturating vapours of o-xylene. …

Stochasticity of TOL plasmid catabolic promoters sets a bimodal expression regime in Pseudomonas putida mt2 exposed to m‐xylene

R Silva‐Rocha, V de Lorenzo - Molecular microbiology, 2012 - Wiley Online Library
… We make the case that this is not a casual occurrence but that such performance encoded
in the TOL network optimizes the adaptation of P. putida mt-2 populations to niches with …

Improving the prediction of Pseudomonas putida mt-2 growth kinetics with the use of a gene expression regulation model of the TOL plasmid

M Koutinas, A Kiparissides, MC Lam… - Biochemical …, 2011 - Elsevier
… Ps/Pr promoters of the TOL (pWW0) plasmid of Pseudomonas putida mt-2, involved in the
… kinetic model was validated with batch cultures of mt-2 fed with m-xylene and succinate and …

The logic layout of the TOL network of Pseudomonas putida pWW0 plasmid stems from a metabolic amplifier motif (MAM) that optimizes biodegradation of m-xylene

R Silva-Rocha, H de Jong, J Tamames… - BMC Systems Biology, 2011 - Springer
… genetic network of the TOL plasmid pWW0 of the soil bacterium Pseudomonas putida mt-2 for
… To gain an insight into the inner layout of this network a logic model of the TOL system was …