… influenza virus by reverse transcription-PCR (RT-PCR). The avian influenza virus is identified
by RT-PCR … nucleoprotein (NP) gene of avian influenza virus. The HA-subtypes of avian …

… RT-PCR was not undertaken on blood samples. In the present study, we … RT-PCR assay
to detect influenza virus RNA. Using this highly sensitive method, it was found that the RT-PCR …

… We also thank the CDC for sharing the influenza virus RT-PCR protocol through a material
transfer agreement. We also thank ZeptoMetrix Corporation for providing influenza virus …

… RT-PCR, providing an increase in detection of influenza viruses of approximately 20%. The
increased detection of influenza virus … Samples which were positive by multiplex RT-PCR but …

… influenza viruses, we used several human influenza virus strains for the entire optimization
process, including an A(H1N1)pdm09 virus (A/New York/1682/2009), an A(H3N2) virus (A/…

… detection for epidemiologic study clinical diagnostic testing for influenza virus. RT-PCR
improves the detection of influenza virus by amplifying even small amounts of viral nucleic acid …

… influenza virus matrix (M) gene–specific PCR primer set and hydrolysis probe were designed
for a region conserved in all type A influenza virus … to North American influenza viruses. …

… types of human influenza viruses, we have developed and evaluated an in-house multiplex
… transcription (RT)-PCR assay for influenza virus A types H1 and H3 and for influenza virus B. …

… hexamers or multiple PCR amplifications with nested primer sets. Our objective was to develop
a rapid molecular surveillance tool to detect influenza viruses in a single PCR reaction. In …

… , type, and subtype type A influenza viruses in a single reverse transcription-PCR. RNA was
… PCR was carried out with a mixture of primers specific for influenza viruses of types B, A/H1, …