Single-cell transcriptional analysis

AR Wu, J Wang, AM Streets… - Annual Review of …, 2017 - annualreviews.org
… , and interpreting a study using single-cell RNA sequencing. … RNA-seq technology with
single-cell transcriptional analysis … of RNA; therefore, it is essential to amplify this RNA to produce …

Transcriptional analysis through RNA sequencing of giant cells induced by Meloidogyne graminicola in rice roots

H Ji, G Gheysen, S Denil, K Lindsey… - Journal of …, 2013 - academic.oup.com
… The goal of our research was to study the transcriptional changes in giant cells formed in
rice roots upon RKN infection. LCM was combined with mRNA sequencing (mRNA-Seq) to …

RNA-seq based transcriptional analysis of Saccharomyces cerevisiae and Lachancea thermotolerans in mixed-culture fermentations under anaerobic conditions

K Shekhawat, H Patterton, FF Bauer, ME Setati - BMC genomics, 2019 - Springer
… The current study aimed to unravel transcriptional responses of S. cerevisiae and L. … allowed
for analysis of early transcriptional responses to mixed culture or analyses of S. cerevisiae …

RNA-seq: from technology to biology

S Marguerat, J Bähler - Cellular and molecular life sciences, 2010 - Springer
… Our understanding of the sequence motifs governing post-transcriptional control improves
steadily but does not yet allow prediction of mRNA processing events based on the genomic …

RNA-seq based transcriptional analysis reveals dynamic genes expression profiles and immune-associated regulation under heat stress in Apostichopus japonicus

D Xu, S Zhou, L Sun - Fish & Shellfish Immunology, 2018 - Elsevier
… Three biological replicates of intestine tissue in each group were prepared for RNA-seq
based transcriptional analysis. Total RNA was extracted using Trizol RNA isolation reagent (…

Dual RNA-seq transcriptional analysis of wheat roots colonized by Azospirillum brasilense reveals up-regulation of nutrient acquisition and cell cycle genes

D Camilios-Neto, P Bonato, R Wassem, MZ Tadra-Sfeir… - BMC genomics, 2014 - Springer
… To our knowledge, this is the first report of a dual RNA-Seq transcriptional analysis of
plant-PGPB interaction (simultaneous analysis of changes in expression of both host and bacteria). …

Lignin metabolism has a central role in the resistance of cotton to the wilt fungus Verticillium dahliae as revealed by RNA-Seq-dependent transcriptional analysis and …

L Xu, L Zhu, L Tu, L Liu, D Yuan, L Jin… - Journal of …, 2011 - academic.oup.com
… changes after pathogen inoculation by RNA-Seq. Of 32 774 genes detected … analyses and
functional assignments of differentially expressed genes indicated a significant transcriptional

A Genome-Wide Transcriptional Analysis of Yeast-Hyphal Transition in Candida tropicalis by RNA-Seq

Y Wu, Y Li, S Yu, W Li, X Liu, L Zhao, J Lu - PloS one, 2016 - journals.plos.org
… In the present study, we firstly carried out a genome-wide transcriptional analysis of three
C. tropicalis isolates which have different adhesion, BF, and hyphal growth abilities under the …

Analysis of intronic and exonic reads in RNA-seq data characterizes transcriptional and post-transcriptional regulation

D Gaidatzis, L Burger, M Florescu, MB Stadler - Nature biotechnology, 2015 - nature.com
… splicing events 24 and later was used to fit a detailed transcriptional model within a single …
proxy for nascent transcription 26 . Here we analyze 17 published RNA-seq data sets covering …

RNA sequencing and analysis

KR Kukurba, SB Montgomery - Cold Spring Harbor Protocols, 2015 - cshprotocols.cshlp.org
… is converted to complementary DNA (cDNA) by reverse transcription and sequencing
adaptors are ligated to the ends of the cDNA fragments. Following amplification by PCR, the …