… ubiquitin E2 enzymes by both bioinformatics and activity assays. To further define … function
of Arabidopsis ubiquitin E2 enzymes, we examined E2-E3 specificity in in vitro ubiquitination …

… to function as Ub E3 ligases and the wheat germ extract contains endogenous E1, E2, and
Ub, we hypothesized that these smears and high molecular mass forms result from Ub ligase …

… by the ubiquitin ligase or E3 enzyme. Uniquely, E3 ligases of the HECT domain class form
a ubiquitin thioester prior to transfer to the substrate, whereas all the other ligases interact …

… At the end of a three-enzyme cascade, ubiquitin ligases … in structure–function studies of
ubiquitin ligases as well as exciting … Our increased understanding of ubiquitin ligase function and …

… Our results showed that the fusion protein SlBAH1 possesses E3 ubiquitin ligase enzyme
activity and that the RING domain in SlBAH1 is essential for this activity. SlBAH1-silencing in …

… As the number of Ub E3 ligases (the third factor in the ubiquitination cascade that determines
target specificity) increased during evolution, so did the number of USPs, suggesting an …

… dictates the type and biological consequence of ubiquitination, it is important to understand
the basis of specificity in E2:E3 interactions. The E3 ligase CHIP interacts with Hsp70 and …

… of this pathway, a ubiquitin-conjugating enzyme (E2), and the activated form is maintained …
of the E2 enzyme. The third enzyme in the process, the ubiquitin protein ligase (E3), supports …

… are not required for its polyubiquitination function, we used its carboxy-… characterization of
these reactions may reveal additional examples of thiol-linked ubiquitin chains on E2 enzymes…

… enzymes (DUBs) function in DSB responses. Here, by carrying out a multidimensional
screening strategy for human DUBs, we identify several with hitherto unknown links to DSB repair, …