… GhALARP as a target gene to evaluate the construction protocol and the editing efficiency
of the 35S:Cas9-sgRNA construct. GhALARP is predominantly expressed in cotton fibers and …

… (GhARG), Gh_A05G2143 and Gh_D05G2397, in the A and D chromosomes that were
mutated with CRISPR/Cas9 in upland cotton R18, a transgenic acceptor variety bred from the …

… ) and CRISPR-associated (Cas) genes, genome editing has opened a new era for
targeted-induced gene mutagenesis and precision breeding in cotton. 2 Genome Editing Tools …

… Although we have used the Agrobacterium-mediated stable transformation to conduct gene
editing in cotton in the current study, it may also be worth exploring particle bombardment-…

… facilitated the development of high-oleic, non-transgenic mutants in allotetraploid upland
cotton with an editing efficiency as high as 54% [29]. These transgene-free mutants hold …

… The first targeted gene mutation was achieved in tobacco plants at the end of the previous
century which involved DSBs. A natural meganuclease I-SceI 18-bp recognition site was used …

… Whether CLCrV-mediated VIGE can achieve gene editing in germ cells of cotton and
obtain mutant seeds for target genes remains to be determined. The plants inoculated with …

… genome editing … target genes is efficient. In this study, we have demonstrated that protein
sequence homology can be a basis for the discovery of many new target genes in upland cotton…

… In the year 2015, the genome of both sea-island and upland cotton was sequenced, … as
VP16/VP64 to activate the expression of target genes with the potential to screen for the gain of …

… In this study, we developed a CRISPR/Cas9-mediated genome editing system in plants
with easy and convenient target sequences, by which the cotton target gene, Gh14-3-3d, were …