… uracil from U:G mispairs in DNA. However, its implications in development of vertebrate
embryos are … In this study, we found that zebrafish uracil-DNA glycosylase a (Unga) is maternally …

… Cell-free extracts from unfertilized eggs and early stage embryos as well as from adult fish
are capable of recognizing and removing uracil in a U/G mispair by uracil DNA glycosylase, …

… uracil may be present and well tolerated in DNA mostly as U:A pairs, likely in quantities of 10
4 per genome. Although a role of uracil in DNA … undetectable in the embryonic stage, most …

… (a) The pathway illustrates the normal development of Ung +/+ B … Eventually, this may lead
to the development of follicular … of the apparently redundant uracil DNA glycosylases TDG and …

… In conclusion, the zebrafish embryo is a potential screening tool to examine DNA methylation
… During the bisulfite treatment only unmethylated cytosines are converted into uracil. The …

… WE have used a forward genetic approach in zebrafish to identify and study genes essential
for retinal development. The neural retina of zebrafish, as in other vertebrates, arises from …

… DNA glycosylase (TDG). Although TDG is essential for active demethylation in embryonic stem
… in mouse zygotes and dispensable in pronuclear DNA demethylation. To search for other …

… level of genomic uracil in primary cells and embryonic fibroblasts from mice deficient in the
… Initial studies revealed that two of these proteins, from zebrafish and Xenopus laevis, had …

… Research in this dissertation shows that DNA damage increases in zebrafish embryos at
the … glycosylase or single-strand-selective monofunctional uracil DNA glycosylase (Tdg/Smug1) …

… It was, therefore, of interest to identify this cryptic uracil–DNA glycosylase. In a parallel
development, a previously unrecognized uracil–DNA glycosylase was identified by an in vitro …