Isolation and characterization of urinary extracellular vesicles: implications for biomarker discovery

ML Merchant, IM Rood, JKJ Deegens… - Nature Reviews …, 2017 - nature.com
… In this Review, we address the evolving nature of the study of urinary extracellular vesicles
… characterization of human urinary extracellular vesicles. Biogenesis of extracellular vesicles

Urinary extracellular vesicles and the kidney: biomarkers and beyond

M Salih, R Zietse, EJ Hoorn - American Journal of …, 2014 - journals.physiology.org
vesicle populations in urine has not been established, we will use the term “urinary extracellular
vesicles” … Although mRNA profiles from whole cells in urine are being studied as disease …

[HTML][HTML] Urinary extracellular vesicles as source of biomarkers in kidney diseases

A Gámez-Valero, SI Lozano-Ramos, I Bancu… - Frontiers in …, 2015 - frontiersin.org
Most cells physiologically release vesicles as way of intercellular communication. The so-called
Extracellular Vesicles (EVs) include exosomes, ectosomes, and apoptotic bodies, which …

Comparing approaches to normalize, quantify, and characterize urinary extracellular vesicles

CJ Blijdorp, OAZ Tutakhel, TA Hartjes… - Journal of the …, 2021 - journals.lww.com
Urinary extracellular vesicles (uEVs) are a promising noninvasive source of kidney … To
address the hypothesis that urine creatinine can be used as a normalization variable, urine

Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles

U Erdbrügger, CJ Blijdorp, IV Bijnsdorp… - … extracellular vesicles, 2021 - Wiley Online Library
Urine is commonly used for clinical diagnosis and … of extracellular vesicles (EV) in urine
opened a new fast-growing scientific field. In the last decade urinary extracellular vesicles (uEVs) …

[HTML][HTML] Urinary extracellular vesicles. A promising shortcut to novel biomarker discoveries

K Barreiro, H Holthofer - Cell and Tissue Research, 2017 - Springer
… The discovery of ubiquitous extracellular vesicles (EV) in all … and findings utilizing urinary
extracellular vesicles as highly … and standardization achieved, urine is foreseen as forming a …

Urinary extracellular vesicles: Origin, role as intercellular messengers and biomarkers; efficient sorting and potential treatment options

P Svenningsen, R Sabaratnam, BL Jensen - Acta Physiologica, 2020 - Wiley Online Library
… Common methods for isolation of urinary extracellular vesicles (uEVs). The enrichment of
EVs (green) from urine samples is based on size, density and surface markers/post-translation …

Isolation and characterization of human urine extracellular vesicles

Z Liu, DM Cauvi, EMA Bernardino, B Lara… - Cell Stress and …, 2018 - Springer
… forms large extracellular filaments co-sedimenting with ECV. We developed a method to
obtain human urine ECV free of UMOD by the addition of ZnSO 4 prior to vesicle isolation by …

Urinary extracellular vesicles: potential biomarkers of renal function in diabetic patients

A Kamińska, M Platt, J Kasprzyk… - Journal of Diabetes …, 2016 - Wiley Online Library
… Typically 50 mL first void urine was used for the isolation of the urinary extracellular
vesicles and processed within 2 h of collection. Samples were centrifuged in a Hermle Z300K (…

[HTML][HTML] Clinical application of human urinary extracellular vesicles in kidney and urologic diseases

G De Palma, F Sallustio, FP Schena - International Journal of molecular …, 2016 - mdpi.com
extracellular vesicles from urine. Increased levels of integrin alpha-3 and integrin beta-1 have
been found in urine … with extracellular vesicles has been found at high levels in urine [92]. …