… two cells … vertebrate model for studying DENV host range evolution, we used the human
hepatoma Huh-7 and Ae. albopictus C6/36 cell lines as surrogate hosts for host range evolution. …

… the ability to form visible plaques in vertebrate cells, and (iii) … replication limits rates of arbovirus
evolution is supported by … to alternating vertebrate-mosquito host cell passage exhibited …

… vertebrate cells. Selection from a pool of mutated promoter sequences appeared to be
stronger in mosquito cells, but passage of the SINV populations in both mosquito and hamster …

… 10 times in vertebrates, mosquitoes or vertebrate cells or alternately passaged 10 times
in vertebrates and mosquitoes. To control for possible phenotypic effects of cell type-specific …

… cell derived virus relative to the vertebrate cells or cycled strains, supporting the idea that, at
least in cell culture, it is replication in invertebrate cells … Given the obvious limitations of cell …

… detected for BHK cells. These results support the hypothesis that arbovirus evolution may be
… However, other studies focusing on vertebrate cells showed fitness declines in unselected …

… cells can constitute similar environments for viral replication. Thus, the slow rates of evolution
observed in natural populations of arboviruses … to both arthropod and vertebrate cell types. …

… Mosquito-specific viruses are restricted in vertebrate cells at multiple levels, … arboviruses
evolved antagonists of the vertebrate interferon pathway, such as the NSs protein of arboviruses …

… Large deletions of DNA close to the left-hand terminus of the genome, characteristic also
of ASF virus maintained continuously in vertebrate cell culture (see above), were observed in …

… vertebrates or in vertebrate cells. These viruses (now totaling at least 64 published) have
genomes analogous to viruses in various families that include arboviruses … to infect vertebrates. …