… purification of AAV1 or AAV4 vectors. Here we describe the purification of rAAV1, 2, and
5 vectors by iodixanol … The resulting vector stocks are 99% pure with titers of 1×10 12 to 1×10 …

… This review discusses general purification methods for viral vectors and vaccines and provides
a … There are currently eight immunologically distinct serotypes of AAV; these vary in their …

… The net charge at neutral pH will depend on the virion surface composition that will differ
for each viral vector type and serotype (Fig. 1). Depending on their net charge, either anion …

… purification. Here we review state of the art methods for AAV vector production and purification,
… is justified by their unique features, which distinguish them from many other viral vector …

… To date, most reported purification schemas are serotype … purification process that is
compatible with the purification of … to characterize recombinant adeno-associated viral vectors. …

… for clinical applications are mainly derived from serotypes 2 and 5. This review describes
production and purification of rAd derived from serotype 5. Serotypes 2 and 5 are associated …

… The choice of iodixanol gradient centrifugation for the downstream process was influenced
by our desire to maintain a generic purification process for all serotypes. The isotonic, …

… platform purification process. Affinity chromatography has proven to be a viable solution
for the purification of viral vectors and to date, a small number of, mostly single serotype-targeted …

… Serotypes other than AAV2 must be purified using alternate procedures 9 . One advantage of
heparin column purification is it is believed to produce AAV vectors … in the purified viral stock…

… The method could be applied to several serotypes and for vectors purified from both …
serotypes to purify. Its been observed previously that the unique N-terminus of the AAV capsid viral …