Virome sequencing of stool samples

L Kramná, O Cinek - The Human Virome: Methods and Protocols, 2018 - Springer
samples. Here, we present a detailed protocol that has been utilized in virome studies where
hundreds of samples … assess the association of the stool virome with susceptibility to type 1 …

Next-generation sequencing of the DNA virome from fecal samples

CL Monaco, DS Kwon - Bio-protocol, 2017 - bio-protocol.org
… a detailed protocol for DNA virome analysis of low input human stool samples (Monaco et
al.… : 1) stool samples are pulverized to evenly distribute microbial matter; 2) stool is enriched for …

Dynamics of the stool virome in very early-onset inflammatory bowel disease

G Liang, MA Conrad, JR Kelsen… - Journal of Crohn's …, 2020 - academic.oup.com
… reads in a typical virome sample are unattributed. Thus, … analysis of the gut virome using
stool samples from a VEO-IBD … contig sequence be covered by the metagenomic virome reads. …

… profiles within the Finnish Diabetes Prediction and Prevention study: Parallel use of 16S profiling and virome sequencing in stool samples from children with islet …

O Cinek, L Kramna, J Lin, S Oikarinen… - Pediatric …, 2017 - Wiley Online Library
… The sequencing data have been deposited at Sequence Read Archive (NCBI) under project
… 2.5 Bioinformatic analysis of the virome The metagenomic data from the virome sequencing

Characterization of stool virome in children newly diagnosed with moderate to severe ulcerative colitis

R Tokarz, JS Hyams, DR Mack, B Boyle… - Inflammatory Bowel …, 2019 - academic.oup.com
… -Vert, a high-throughput sequencing virus capture platform, to examine the stoolstool virome
in IBS, an altered microbiome has been reported in IBS. We only analyzed stool specimens

Gut virome sequencing in children with early islet autoimmunity

L Kramná, K Kolářová, S Oikarinen… - Diabetes …, 2015 - Am Diabetes Assoc
… The stool virome in 19 case children, who turned islet autoantibody positive before the age
… control subjects was analyzed using NGS performed from stool samples collected 3, 6, and 9 …

A protocol for extraction of infective viromes suitable for metagenomics sequencing from low volume fecal samples

L Deng, R Silins, JL Castro-Mejía, W Kot, L Jessen… - Viruses, 2019 - mdpi.com
sample input yielding purified viromes containing phages that are still infective, which also
are of sufficient purity for genome sequencing. … 1–3refer to viromes extracted from feces from …

[HTML][HTML] Emerging view of the human virome

KM Wylie, GM Weinstock, GA Storch - Translational research, 2012 - Elsevier
… in virome analysis, which has been driven by advances in high-throughput, deep sequencing
… in a human stool sample, shotgun sequencing of 532 cloned viral DNA fragments from the …

The human intestinal virome in health and disease

SR Carding, N Davis, L Hoyles - Alimentary pharmacology & …, 2017 - Wiley Online Library
… Deep sequencing of faecal viromes (48 Gb of virome DNA across 12 … viromes obtained from
faecal samples at weeks 1 and 2 of life with probes constructed from the week 1 sequencing

The human virome: assembly, composition and host interactions

G Liang, FD Bushman - Nature Reviews Microbiology, 2021 - nature.com
Virome sequencing studies have unveiled some new lineages of human viruses that appear
to … A later study of neonate stool samples, investigating both RNA and DNA viromes, taken a …