… The electron (e − ) and ion (Ga + ) beam are placed at 52 … beam, and –7 to the focused
ion beam. c and d, Schematic drawing of a cell attached to the grid loaded inside the dual-beam …

… , making reconstruction of an entire eukaryotic cell technically very challenging. In an … using
FIB-SEM for whole cell reconstructions, we pushed the limits of focused ion beam milling and …

… Historically, investigations into the 3D ultrastructure of relatively large biological samples
such as whole cells and tissues have used serial-section TEM (ssTEM), in which sequential …

… of cellular ultrastructure. In work carried out recently,13 we have attempted a comparative …
into whole cell microscopy using fast and slow ions. Slow (eg 10À50 keV) helium ion beams …

… For microscopy, a highly focused MeV ion beam can pass through thin sections eg a whole
single cell, without significant loss of spatial resolution, and produces only short range lateral …

… important to prepare the cultured cells in such a way that their ionic integrity is preserved, …
cell is damaged by the high energy focused ion beam. Preparation of individual cultured cells …

… of the cell-to-material interface at any desired location, based on focused ion beam milling
… For image acquisition of whole cells (i.e., Figure 1b) a secondary electron detector was used…

… an ion beam which can be oriented and focussed on the sample. The focussed ion beam is
… that all the available current methods give an information that is averaged on the whole cell. …

… cells or tissues is the accessible specimen thickness. Recently it has been shown that
cryo-focused ion beam milling of plunge-frozen eukaryotic cells … images of the whole grid based …

… all kinds of cells grown or deposited directly on EM grids. With the ion beam incident under
a … Because the whole procedure is carried out on the EM grid, transfer to the TEM cryoholder …