… PCR), which can rapidly assess the methylation status of virtually any group of CpG sites within
a CpG island… This assay entails initial modification of DNA by sodium bisulfite, converting …

… , followed by PCR reactions. Here we describe a method, methylated-CpG island recovery
assay (… Matrix-assisted binding and simple PCR assays are used to detect methylated DNA …

… -PCR assay was used to study DNA methylation in individual mouse embryos. It was found
that HpaII site H-7 in the CpG island … An assay based on the use of HpaII prior to the polymer…

… 7 for hTERT gene expression using RT-PCR. The autoradiographs are overexposed to
show the induced hTERT expression; the RT-PCR assay was designed to be linear for hTERT …

… CpG island. To examine more sites and increase sensitivity, we developed a methylation-…
PCR assay to map methylation of the entire ER CpG island. The island was unmethylated in …

… cells displayed abnormal GSTPl 'CpG island' hypermethylation. … 'CpG island' DNA
hypermethylation and restored GSTPl expression. Using a PCR assay, somatic GSTPl 'CpG island' …

… PCR assay to assess methylation, we were able to demonstrate that the degree of CpG island
… promoter core domain, our PCR assay focused on this region, which contains one CpG …

… Examples of HpaII-McrBC PCR assays for CpG islands on human Chromosome 21. The
results of self-Harr plot and HpaII-McrBC PCR were depicted for (A) a completely methylated …

… We reviewed the literature to evaluate CpG island … with respect to CpG island
hypermethylation patterns in other … For restriction enzymes PCR assays, the PCR amplicon is …

… RT-PCR assay confirmed that WT1 and PITX2 were only weakly expressed in the breast
cancer tissues and were not expressed in most non-cancerous breast tissues. OTX2 and PAX6 …