… RT-PCR assay confirmed that WT1 and PITX2 were only weakly expressed in the breast
cancer tissues and were not expressed in most non-cancerous breast tissues. OTX2 and PAX6 …

… controls in real-time PCR or performing endpoint PCR amplification, we can … CpG island
is an unmethylated region in all cells, we used it as a general negative control in our assays. …

… were further tested using an independent set of 149 samples from colorectal adenocarcinomas,
other diseases and healthy tissues, using a quantitative methylation-specific PCR assay. …

… the arraying of CpG island clones on glass slides, the preparation of the sample amplicons
under investigation, and the hybridization of amplicon targets onto the CpG island microarray. …

… A TaqMan-based real-time PCR assay was performed to detect the methylation levels of the
SOX9 gene in 156 cervical scrapings, including 48 normal cervical scrapings, 30 scrapings …

… Another modification that we have adapted to the ChIP assay that differs from our published
protocols is the use of Qiaquick PCR purification columns (Qiagen, 28104) in lieu of the …

… Incomplete methylation-sensitive digestion might occur during sample preparation, resulting
in false-positive detection of DNA methylation after PCR amplification. We therefore tested …

… So far, epigenomic studies have succeeded in identifying CpG islands, but recent evidence
points towards a role for transposable elements in epigenetic regulation, causing the fields of …

… (b) methylation detection does not require PCR amplification or DNA methylase … assay is
demonstrated by the detection of an increase in genome-wide hypomethylation and CpG island …

… To isolate differentially methylated CpG islands in cancer and … for the efficient PCR
amplification of methylated CpG islands, … a novel PCR assay for methylation status of CpG islands. …