Enhanced sensitivity of CpG island search and primer design based on predicted CpG island position
HC Park, ER Ahn, JY Jung, JH Park, JW Lee… - Forensic Science …, 2018 - Elsevier
… PCR to identify the methylation of particular CpG sites. In this study, we performed CpG island
… chromosomes and compared CpG island search performance of CpGPNP with other CpG …
… chromosomes and compared CpG island search performance of CpGPNP with other CpG …
CpG-island promoters drive transcription of human telomeres
SG Nergadze, BO Farnung, H Wischnewski, L Khoriauli… - Rna, 2009 - rnajournal.cshlp.org
… We then PCR-amplified the obtained cDNA with unique primer pairs corresponding to … 3C)
and sequence (data not shown) the ones obtained in PCR reactions performed on genomic …
and sequence (data not shown) the ones obtained in PCR reactions performed on genomic …
Effect of CpG island methylation on microRNA expression in the k-562 cell line
Y Yang, LL Wang, YH Li, XN Gao, Y Liu, L Yu - Biochemical genetics, 2012 - Springer
… CpG island is associated with regulation of microRNA expression, we investigated CpG islands
… whether the CpG islands were methylated by methylation-specific PCR in the k-562 cell …
… whether the CpG islands were methylated by methylation-specific PCR in the k-562 cell …
Methylation profiles of genes utilizing newly developed CpG island methylation microarray on colorectal cancer patients
N Kimura, T Nagasaka, J Murakami… - Nucleic acids …, 2005 - academic.oup.com
… Genomic DNA was treated with bisulfite and amplified for a specific region of interest containing
multiple CpG islands by PCR. PCR product was labeled with biotin and hybridized to the …
multiple CpG islands by PCR. PCR product was labeled with biotin and hybridized to the …
Methylation analysis of CpG islands
R Lilischkis, H Kneitz, H Kreipe - … Protocols: Volume I: Analysis of Cells …, 2001 - Springer
… Whereas CpGs found in the bulk of genomic DNA are usually methylated, CpGs in CpG
islands … The PCR primers are chosen to bind specifically to bisulfite converted DNA in stretches …
islands … The PCR primers are chosen to bind specifically to bisulfite converted DNA in stretches …
Multiplexed profiling of candidate genes for CpG island methylation status using a flexible PCR/LDR/Universal Array assay
YW Cheng, C Shawber, D Notterman, P Paty… - Genome …, 2006 - genome.cshlp.org
… in CpG islands is associated with transcriptional silencing. Accurate determination of cytosine
methylation status in promoter CpG … a quantitative PCR/LDR/Universal Array assay that …
methylation status in promoter CpG … a quantitative PCR/LDR/Universal Array assay that …
CpG island methylation in sporadic colorectal cancers and its relationship to microsatellite instability
N Hawkins, M Norrie, K Cheong, E Mokany, SL Ku… - Gastroenterology, 2002 - Elsevier
… CpG islands is increasingly recognized as an important event in colorectal carcinogenesis.
We evaluated the extent of CpG island … -specific polymerase chain reaction (PCR), whereas …
We evaluated the extent of CpG island … -specific polymerase chain reaction (PCR), whereas …
[HTML][HTML] Initiation of DNA replication at CpG islands in mammalian chromosomes
… as input for PCR. Electrophoresed … PCR products from each fraction hybridised to internal
primers as in Figure 1. (B) Quantitative measurement by competitive PCR of the CpG island, …
primers as in Figure 1. (B) Quantitative measurement by competitive PCR of the CpG island, …
E-cadherin expression is silenced by 5′ CpG island methylation in acute leukemia
PG Corn, BD Smith, ES Ruckdeschel, D Douglas… - Clinical cancer …, 2000 - AACR
… In this study, we used a nested-PCR approach to examine the methylation status of the E-cadherin
5′ CpG island in blood and bone marrow samples from normal donors and in bone …
5′ CpG island in blood and bone marrow samples from normal donors and in bone …
[HTML][HTML] Aberrant CpG island hypermethylation of multiple genes in colorectal neoplasia
S Lee, KS Hwang, HJ Lee, JS Kim, GH Kang - Laboratory investigation, 2004 - Elsevier
… CpG island methylation is an early and frequent event during colorectal carcinogenesis. It
appears that CpG island … methylated PCR products showed extensive methylation of CpG sites …
appears that CpG island … methylated PCR products showed extensive methylation of CpG sites …