Detailed methylation analysis of CpG islands on human chromosome region 9p21

RJ Weeks, IM Morison - Genes, Chromosomes and Cancer, 2006 - Wiley Online Library
… /HhaI/HpaII PCR results, it would appear that the PCR products in the HhaI- … -PCR, and
COBRA, fail to reveal the complexity of the heterogeneous methylation patterns in CpG islands. …

Roles of cell division and gene transcription in the methylation of CpG islands

CM Bender, ML Gonzalgo, FA Gonzales… - … and cellular biology, 1999 - Am Soc Microbiol
… 2 CpG island after 5-aza-2′-deoxycytidine (5-Aza-CdR) treatment occurs more rapidly than
that of the p16 promoter CpG island. … A 149-bp region (hatching) was amplified by PCR with …

Silencing of GSTP1 Gene by CpG Island DNA Hypermethylation in HBV-associated Hepatocellular Carcinomas

S Zhong, MW Tang, W Yeo, C Liu, YMD Lo… - Clinical cancer …, 2002 - AACR
… Because the prevalence of the hypermethylation in tumor and nontumor tissues, as
revealed in this study, is different, PCR-based strategies targeting GSTP1 CpG island DNA …

… vivo footprint and methylation analysis by PCR-aided genomic sequencing: comparison of active and inactive X chromosomal DNA at the CpG island and promoter of …

GP Pfeifer, RL Tanguay, SD Steigerwald… - Genes & …, 1990 - genesdev.cshlp.org
… Our results extend the presently available data on DNA methylation m CpG islands to all
CpG dinucleotides in the PGK-1 promoter region. Not only are restriction sites unmethylated on …

Expressed CpG island sequence tag microarray for dual screening of DNA hypermethylation and gene silencing in cancer cells

H Shi, PS Yan, CM Chen, F Rahmatpanah… - Cancer Research, 2002 - AACR
We present a novel concept by using expressed CpG island sequence tags (ECISTs)for dual
analysis of DNA methylation and gene expression in cancer cells. ECISTs are present in …

Susceptibility of Nonpromoter CpG Islands to De Novo Methylation in Normal and Neoplastic Cells

C Nguyen, G Liang, TDT Nguyen… - Journal of the …, 2001 - academic.oup.com
… that exonic CpG islands are more susceptible to de novo methylation than promoter islands
and that … Purified bisulfite-specific PCR products of each of the six CpG islands were used as …

Unusual distribution of DNA methylation within the hTERT CpG island in tissues and cell lines

I Guilleret, J Benhattar - Biochemical and biophysical research …, 2004 - Elsevier
… In telomerase-positive samples, a methylation of all the CpG … In conclusion, hypermethylation
of the CpG island plays a … PCR products analyzed in this study (P1–P5 and G1–G5) are …

DNA methylation directly silences genes with non-CpG island promoters and establishes a nucleosome occupied promoter

H Han, CC Cortez, X Yang, PW Nichols… - Human molecular …, 2011 - academic.oup.com
island promoters display a nucleosome-depleted … -CpG island promoters are almost identical
to CpG island promoters, suggesting that aberrant methylation patterns of non-CpG island

CpG island hypermethylation in human colorectal tumors is not associated with DNA methyltransferase overexpression

CA Eads, KD Danenberg, K Kawakami, LB Saltz… - Cancer research, 1999 - AACR
… RT-PCR or DNA methylation studies yield values that are expressed as ratios between two
absolute measurements (DNMT:normalization gene for RT-PCR and CpG island:MYOD1 for …

Expression of mRNA for DNA methyltransferases and methyl-CpG–binding proteins and DNA methylation status on CpG islands and pericentromeric satellite regions …

Y Saito, Y Kanai, M Sakamoto, H Saito, H Ishii… - Hepatology, 2001 - Elsevier
… In this analysis, a PCR product in the primer sets M and U reflects the presence of
methylated and … Methylationspecific PCR: a novel PCR assay for methylation status of CpG