… The RT-PCR assay was performed as described previously (32) by coamplification of 0.3 g
of total RNA and a known amount of competitor RNA. Primers used were designed to amplify …

… The primer sequences and PCR conditions of the other genes are given in the … bi-
sulphite-modified sequences of CpG island loci, we used a PCR mixture containing 1 × PCR buffer, …

… the CpG islands in the two genes. The microarray data were further confirmed by
methylation-specific PCR … , Methylation-specific PCR: A novel PCR assay for methylation status of …

… 2 to 4 CpG sites within an island region. CpG island hypermethylation assayed by Southern
… or, more recently, with polymerase chain reaction (PCR)-based approaches based on …

… genes have 5′ CpG islands which are constitutively … by a wave of non-CpG island de
novo methylation that occurs at … key role in protecting a CpG island in the adenine phosphor!…

… Our preliminary analysis showed that purification of PCR products was not a prerequisite for
… PCR products at a final concentration of 20% to allow for uniform dot morphology. The PCR …

… methylation was detected by the PCR assay in these cells up … CpG island methylation were
detected. In E6-expressing HMEC6, methylation was first readily detected by the PCR assay …

Background & Aims: The CpG island methylator phenotype (CIMP) is one of the mechanisms
involved in colorectal carcinogenesis (CRC). Although CIMP is probably the cause of high-…

… Loss of a marker was considered to be present when the PCR assay showed absence or
decrease in intensity by more than 50% of a band from a tumor sample as compared with the …

… CpG-enriched fraction was eluted by using Qiaquick PCR purification kits (Qiagen,
Valencia, CA). Eluted fractions or MseI-digested and linker-ligated input fractions were PCR-amplified …