Culture conditions for human embryonic stem cells

H Skottman, O Hovatta - Reproduction, 2006 - rep.bioscientifica.com
… , but also for reliable research carried out using these cells. It is difficult to evaluate the …
culture conditions on cells. Hence, a defined culture system with known composition of culture

Defined culture conditions of human embryonic stem cells

J Lu, R Hou, CJ Booth, SH Yang… - Proceedings of the …, 2006 - National Acad Sciences
… To further optimize the hESC culture conditions, we tested a variety of different components
and found that the addition of chemically defined cholesterol to the medium improved hESC …

Optimizing stem cell culture

B Van Der Sanden, M Dhobb… - Journal of cellular …, 2010 - Wiley Online Library
cells, suggests that current stem cell culture conditions … , culture conditions can significantly
influence stem cell fate. A third type of stem cells named induced pluripotent stem (iPS) cells

Characterization of the optimal culture conditions for clinical scale production of human mesenchymal stem cells

PA Sotiropoulou, SA Perez, M Salagianni… - Stem …, 2006 - academic.oup.com
… this study, by comparing various culture conditions, to identify the … culture parameters,
including basal medium, glucose concentration, stable glutamine, bone marrow mononuclear cell

Functional heterogeneity of mesenchymal stem cells from natural niches to culture conditions: implications for further clinical uses

LA Costa, N Eiro, M Fraile, LO Gonzalez, J Saá… - Cellular and Molecular …, 2021 - Springer
… Mesenchymal stem cells (MSC) are present in all organs and tissues. … the conditions of
potential donors (age, diseases or unknown factors). It is accepted that many culture conditions of …

Hypoxic culture conditions as a solution for mesenchymal stem cell based regenerative therapy

N Haque, MT Rahman, NH Abu Kasim… - The Scientific World …, 2013 - Wiley Online Library
… ) conditions (usually 2–9% O 2 concentration) on stem cellculture in ambient and hypoxic
conditions. Finally this paper also highlights how MSCs cultured in vitro in hypoxic conditions

[HTML][HTML] Totipotent embryonic stem cells arise in ground-state culture conditions

SM Morgani, MA Canham, J Nichols, AA Sharov… - Cell reports, 2013 - cell.com
… We asked whether HV + and HV − cells derived from serum/LIF or 2i/LIF culture conditions
represented equivalent cell types by carrying out RNA sequencing (RNA-seq) genome-wide …

[HTML][HTML] Optimization of mesenchymal stem cell expansion procedures by cell separation and culture conditions modification

S Carrancio, N López-Holgado… - Experimental …, 2008 - Elsevier
Cells were cultured in standard conditions (10% fetal calf … obtained with the same culture
conditions to which platelet lysate (… ) until the fourth cell passage. Time of expansion, number of …

Culture conditions and single growth factors affect fate determination of mouse spermatogonial stem cells

H Kubota, MR Avarbock, RL Brinster - Biology of reproduction, 2004 - academic.oup.com
… A major challenge still remaining is to establish a defined serum-free culture condition that
supports maintenance of the stem cell and allows definitive experiments to analyze the effect …

Effects of hypoxic culture conditions on umbilical cord-derived human mesenchymal stem cells

A Lavrentieva, I Majore, C Kasper, R Hass - Cell communication and …, 2010 - Springer
… or lymphatic channels providing conditions that UC cells could also … In this study we therefore
tested the effects of hypoxic conditions (… and metabolic activities in these human stem cells. …