A modified and improved method for bisulphite based cytosine methylation analysis

A Olek, J Oswald, J Walter - Nucleic acids research, 1996 - academic.oup.com
… We are currently performing methylation analyses of larger chromosomal regions and
succeeded in amplifying fragments of up to 3 kb. All our previous attempts to amplify fragment >1 …

RNA cytosine methylation analysis by bisulfite sequencing

M Schaefer, T Pollex, K Hanna, F Lyko - Nucleic acids research, 2009 - academic.oup.com
analysis of RNA methylation patterns we have established a protocol for the chemical
deamination of cytosines … Using tRNAs and rRNAs as examples we show that cytosine methylation

Cytosine methylation and mammalian development

CP Walsh, TH Bestor - Genes & development, 1999 - genesdev.cshlp.org
cytosine methylation in nonexpressing tissues. DNA blot analysis after cleavage with
methylation-… Genes were selected for analysis on the basis of well-characterized promoters, strict …

Advances in the profiling of DNA modifications: cytosine methylation and beyond

N Plongthongkum, DH Diep, K Zhang - Nature Reviews Genetics, 2014 - nature.com
Cytosine methylation has an important role in the regulation of mammalian gene expression.
Additional forms of cytosine … have enabled the use of this approach in single-cell analysis. …

MSAP markers and global cytosine methylation in plants: a literature survey and comparative analysis for a wild‐growing species

C Alonso, R Pérez, P Bazaga… - Molecular Ecology …, 2016 - Wiley Online Library
cytosine methylation levels in plants. Here, we analyse the relationship between MSAP
results and the percentage of global cytosine methylation in genomic DNA obtained by HPLC …

Finding the fifth base: genome-wide sequencing of cytosine methylation

R Lister, JR Ecker - Genome research, 2009 - genome.cshlp.org
… Dramatic developments in high-throughput sequencing technologies are driving a
paradigm shift in global single-base resolution DNA methylation analysis. Several “next-generation” …

Cytosine methylation profiling of cancer cell lines

M Ehrich, J Turner, P Gibbs, L Lipton… - Proceedings of the …, 2008 - National Acad Sciences
… All bisulfite-based methylation analysis methods suffer from a considerable amount of
measurement … to results from a recent methylation study of colon cancer tumors that analyzed DNA …

Optimized design and data analysis of tag-based cytosine methylation assays

M Suzuki, Q Jing, D Lia, M Pascual, A McLellan… - Genome biology, 2010 - Springer
… know the degree of change in cytosine methylation in the disease of … for cytosine methylation
assays that can detect methylationcytosine methylation have addressed this issue, with the …

Cytosine Methylation: Quantitation by Automated Genomic Sequencing and GENESCANTM Analysis

CL Paul, SJ Clark - Biotechniques, 1996 - Taylor & Francis
… The method relies on the ability of sodium bisulfite to efficiently deaminate cytosine residues
… have been amplified as cytosine (C) (3). Analysis of cytosine methylation from the bisulfite-…

Effects of cytosine methylation on transcription factor binding sites

YA Medvedeva, AM Khamis, IV Kulakovskiy… - BMC genomics, 2014 - Springer
… ), we performed the same analysis using the standard PWM … We also analyzed if cytosines
with significant positive SCC M/… In the analysis of TFBSs affected by cytosine methylation, we …