A modified and improved method for bisulphite based cytosine methylation analysis
A Olek, J Oswald, J Walter - Nucleic acids research, 1996 - academic.oup.com
… We are currently performing methylation analyses of larger chromosomal regions and
succeeded in amplifying fragments of up to 3 kb. All our previous attempts to amplify fragment >1 …
succeeded in amplifying fragments of up to 3 kb. All our previous attempts to amplify fragment >1 …
RNA cytosine methylation analysis by bisulfite sequencing
M Schaefer, T Pollex, K Hanna, F Lyko - Nucleic acids research, 2009 - academic.oup.com
… analysis of RNA methylation patterns we have established a protocol for the chemical
deamination of cytosines … Using tRNAs and rRNAs as examples we show that cytosine methylation …
deamination of cytosines … Using tRNAs and rRNAs as examples we show that cytosine methylation …
Cytosine methylation and mammalian development
… cytosine methylation in nonexpressing tissues. DNA blot analysis after cleavage with
methylation-… Genes were selected for analysis on the basis of well-characterized promoters, strict …
methylation-… Genes were selected for analysis on the basis of well-characterized promoters, strict …
Advances in the profiling of DNA modifications: cytosine methylation and beyond
… Cytosine methylation has an important role in the regulation of mammalian gene expression.
Additional forms of cytosine … have enabled the use of this approach in single-cell analysis. …
Additional forms of cytosine … have enabled the use of this approach in single-cell analysis. …
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- cytosine methylation profiling
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MSAP markers and global cytosine methylation in plants: a literature survey and comparative analysis for a wild‐growing species
… cytosine methylation levels in plants. Here, we analyse the relationship between MSAP
results and the percentage of global cytosine methylation in genomic DNA obtained by HPLC …
results and the percentage of global cytosine methylation in genomic DNA obtained by HPLC …
Finding the fifth base: genome-wide sequencing of cytosine methylation
… Dramatic developments in high-throughput sequencing technologies are driving a
paradigm shift in global single-base resolution DNA methylation analysis. Several “next-generation” …
paradigm shift in global single-base resolution DNA methylation analysis. Several “next-generation” …
Cytosine methylation profiling of cancer cell lines
… All bisulfite-based methylation analysis methods suffer from a considerable amount of
measurement … to results from a recent methylation study of colon cancer tumors that analyzed DNA …
measurement … to results from a recent methylation study of colon cancer tumors that analyzed DNA …
Optimized design and data analysis of tag-based cytosine methylation assays
… know the degree of change in cytosine methylation in the disease of … for cytosine methylation
assays that can detect methylation … cytosine methylation have addressed this issue, with the …
assays that can detect methylation … cytosine methylation have addressed this issue, with the …
Cytosine Methylation: Quantitation by Automated Genomic Sequencing and GENESCANTM Analysis
CL Paul, SJ Clark - Biotechniques, 1996 - Taylor & Francis
… The method relies on the ability of sodium bisulfite to efficiently deaminate cytosine residues
… have been amplified as cytosine (C) (3). Analysis of cytosine methylation from the bisulfite-…
… have been amplified as cytosine (C) (3). Analysis of cytosine methylation from the bisulfite-…
Effects of cytosine methylation on transcription factor binding sites
… ), we performed the same analysis using the standard PWM … We also analyzed if cytosines
with significant positive SCC M/… In the analysis of TFBSs affected by cytosine methylation, we …
with significant positive SCC M/… In the analysis of TFBSs affected by cytosine methylation, we …