… denaturation analysis of methylation differences method detects differentially methylated …
To determine whether the common signals in the current analysis were due to methylation or …

… Using a mini-57 bp amplicon, differential amplification dependent on 3 CpG sites could be
demonstrated with a 2.5C difference in denaturation temperature. Since selection against non-…

… To determine genome-wide methylation content, genomic DNA is denatured and … differences
between methylated and unmethylated alleles can then be resolved by denaturation of the …

… We describe two methods that resolve differentially methylated DNA sequences on the
basis of differences in melting temperature. The first … Denaturing gradient gel electrophoresis …

… methylation evaluation with single-base resolution. Despite the advantages of these
approaches, DNA methylation differences … DNA must be fully denatured (single stranded) to ease …

… as great as real differences in genome-wide methylation. The … methylation is present in
more than one method of analysis. … reaction, where incomplete denaturation or partial reaction …

… , methylated and unmethylated, PCR products show different melting profiles when subjected
to thermal denaturation. … These algorithms can also be used to investigate the difference in …

… Rapid quantitation of methylation differences at specific sites using methylationsensitive …
a methylated DNA binding column and denaturing gradient gel electrophoresis. Proc. Natl. …

… An analytical method for the detection of methylation differences at specific chromosomal
loci using primer extension and ion pair reverse phase HPLC. Hum Mutat 2002; 20: 305–11. …

… , combined bisulfite restriction analyses and methylation-sensi… on differential methylation
states (eg, methylationspecific … combines methylation-specific PCR and denaturing HPLC (…