… denaturation analysis of methylation differences method detects differentially methylated …
To determine whether the common signals in the current analysis were due to methylation or …

… Using a mini-57 bp amplicon, differential amplification dependent on 3 CpG sites could be
demonstrated with a 2.5C difference in denaturation temperature. Since selection against non-…

… To determine genome-wide methylation content, genomic DNA is denatured and … differences
between methylated and unmethylated alleles can then be resolved by denaturation of the …

… We describe two methods that resolve differentially methylated DNA sequences on the
basis of differences in melting temperature. The first … Denaturing gradient gel electrophoresis …

… methylation evaluation with single-base resolution. Despite the advantages of these
approaches, DNA methylation differences … DNA must be fully denatured (single stranded) to ease …

… as great as real differences in genome-wide methylation. The … methylation is present in
more than one method of analysis. … reaction, where incomplete denaturation or partial reaction …

… the DNA methylation differences of hypomethylated DMRs. … denaturing step prior to
immunoprecipitation. That is, the CpG dense fragments either do not undergo complete denaturation …

… , and relative methylation is calculated based on the difference of their Ct … of the analysis
for heterogeneous samples, methylation-… Currently, it requires the use of denaturing gradient …

… The resulting modified sequences differ markedly from the original genomic sequence, …
alleles differ at a number of positions corresponding to differentially methylated cytosines in …

… An analytical method for the detection of methylation differences at specific chromosomal
loci using primer extension and ion pair reverse phase HPLC. Hum Mutat 2002; 20: 305–11. …