… the original CpG sites can be easily determined by incubating the gel-isolated PCR product,
primer(s), and Taq polymerase with either [ 32 P]dCTP or [ 32 P]TTP followed by denaturing …

… The resulting modified sequences differ markedly from the original genomic sequence, …
alleles differ at a number of positions corresponding to differentially methylated cytosines in …

… Initial denaturation was performed at 94C for 2 min, followed by five cycles under low-stringency
conditions of 94C for 30 s, 40C for 60 s, 72C for 90 s, and then 30 cycles under higher …

… (2010) DNA methylation of developmental genes in pediatric medulloblastomas identified
by denaturation analysis of methylation differences. Proceedings of the National Academy of …

… denaturing regions of the gradient gel and results in focusing of the band. This property can
be applied to detect the difference in melting temperature between methylated … methylation …

… bisulfiteconverted methylation differences inbetween the two primers can be analyzed by any
… DNA is first denatured by as the ratio of polymerase to DNA template is reduced (phase 3). …

… , and relative methylation is calculated based on the difference of their Ct … of the analysis
for heterogeneous samples, methylation-… Currently, it requires the use of denaturing gradient …

… PCR was initiated by incubation for 1 min at 95 C to denature the TaqStart Antibody, … of
uracils to thymine, methylated and unmethylated alleles are predicted to differ in thermal stability …

… this method for the quantification of DNA methylation differences at specific CpG sites. In …
or [ 32 P] dTTP followed by denaturing polyacrylamide gel electrophoresis and phosphorimage …

… the DNA methylation differences of hypomethylated DMRs. … denaturing step prior to
immunoprecipitation. That is, the CpG dense fragments either do not undergo complete denaturation …