… With successful thermal denaturation profile differences observed for methylated,
hemimethylated, and nonmethylated fragments (Figure 1), we next tested the feasibility of …

… -wide library constructions intended for DNA methylation analysis by alternative high-throughput …
to reveal noticeable differences despite the aforementioned differences in amplification …

… In the current study, we used a denaturing high-performance … to analyze the methylation of
differentially methylated regions (… denaturing conditions and the magnitude of the differences …

… liquid chromatographic analysis of DNA … -specific differences in the extent of methylation
of their cytosine residues. Several similarities in the patterns of tissue-specific DNA methylation …

… denaturation since sodium bisulfite can only react with cytosine in single-stranded DNA.
Therefore, complete DNA denaturation … (6), genomic DNA is denatured in high sodium bisulfite …

… if it is either methylated or in double stranded DNA. Thus the DNA should be denatured prior
to … or unmethylated products taking advantage of the sequence differences resulting after …

… Initial denaturation at 94 C for 2 min, followed by five cycles at … Initial denaturation is for 5
min at 94 C and 34 cycles at low-… of formamide–dye buffer and denatured by heating at 90 C for …

… Especially, incomplete denaturation of the double-stranded DNA before conversion leads to
… , as the sequence difference of formerly methylated and unmethylated molecules induced …

… As demonstrated in Figure 4a, we observed consistent differences in DNA methylation of all
… After denaturation DNA was then immunoprecipitated using 1 µg of mouse monoclonal anti-…

… that differ by a single base-pair can be separated by denaturing … Base modifications such
as methylation are also known to affect … in denaturing gradient gels. The presence of a single …