… In this work we investigate differences between these two techniques in the assessment of
… the methylation percentage of a sequence or site, we recommend that the denaturing step …

… differ in mC distribution. Repetitive sequences usually contain more mC than genic sequences
[9]. Methylation of … that are separated in denaturing polyacrylamide gels. Visualization of …

… technique is based on differences in the affinity of double- … in differences in elution characteristics
of the material analyzed… fragment analyzed at a given partially denaturing temperature, …

… The PCR had an initial denaturation at 94C for 9 min, 10 … The PCR had an initial denaturation
at 94C for 9 min, 10 cycles … have multiple nucleotide differences that will favor the adoption …

… methylation patterns of the three tissues. Significant differences in cytosine methylation level
… The entire mixture was loaded onto a 4.5% denaturing polyacrylamide gel. Electrophoresis …

… -based methylation-analysis technique described by Frommer et al. (10). As a main difference
… DNA strands after the following denaturation. Agarose-embedded (and -digested) DNA is …

… Our results show that Heat denaturation yielded best conversion, whereas Alkaline … number
of methylation differences, showing that our analysis is picking up relevant differences that …

… tray containing denaturation buffer and count to 10 as above. Amplicons are denatured and
… in efficiencies may skew results, potentially masking or overestimating differences between …

… N′-[3-propanesulfonic acid], pH 8.0), denatured at 96C for 10 min, and reannealed at 67C
for 16… Denaturing, reannealing, and selective amplification of the self-annealed product were …

… DNA after the initial denaturation step, several protocols introduced a denaturing step (95 C,
… is the separate analysis and quantification of sequences showing methylation differences in …