Methylation of somatic vs germ cell DNAs analyzed by restriction endonuclease digestions

J Kaput, TW Sneider - Nucleic acids research, 1979 - academic.oup.com
… M in NaCl by adding solid NaCl, denatured at 75C for 30 minutes, and … different methylation
patterns 1n bovine DNA as compared to Novikoff rat hepatoma DNA and to the methylation

The methylation status of plant genomic DNA influences PCR efficiency

KV Kiselev, AS Dubrovina, AP Tyunin - Journal of plant physiology, 2015 - Elsevier
methylated plant DNA regions. We suggest that increased DNA denaturation temperatures
of the highly methylated … cycles of amplification, since the difference in PCR product yield for …

Comparison of genome‐wide analysis techniques to DNA methylation analysis in human cancer

N Soozangar, MR Sadeghi, F Jeddi… - Journal of cellular …, 2018 - Wiley Online Library
… In this technique, following sonication, DNA fragments are denatured by heat treatment and
… This technique has also been used to analyze differences in CpG site methylation in DNA …

Differences in the binding of methylated albumin to non-replicating, replicating and denatured DNA from Ehrlich ascites cells

T Hofstaetter, H Probst - Biochimica et Biophysica Acta (BBA)-Nucleic …, 1978 - Elsevier
… ], and since denatured DNA is eluted from … methylated albumin to replicating DNA in the
test tube and compared the results with those obtained with fully double-stranded and denatured

Analysis of DNA methylation during germination of pepper (Capsicum annuum L.) seeds using methylation-sensitive amplification polymorphism (MSAP)

E Portis, A Acquadro, C Comino, S Lanteri - Plant Science, 2004 - Elsevier
… , and significant differences in cytosine methylation levels have … Here, we report on the
analysis of cytosine methylation … and 0.01% w/v xylene cyanol), denatured at 95 C for 4 min and …

A study of chemically methylated deoxyribonucleic acid

J Ramstein, C Hélène, M Leng - European Journal of …, 1971 - Wiley Online Library
… The difference between effects in vivo and in vitro has been ascribed to … methylated DNA
is not locally denatured. Stacking of methylated residues still occurs in denatured methylated

Genome-wide analysis of DNA methylation patterns

D Zilberman, S Henikoff - 2007 - journals.biologists.com
… DNA is denatured and then treated with sodium … methylated DNA. Although less of an issue
with McrBC, sequence polymorphisms between samples can mimic methylation differences if …

Enrichment of Methylated Molecules Using Enhanced-Ice-Co-Amplification at Lower Denaturation Temperature-PCR (E-Ice-COLD-PCR) for The Sensitive Detection …

F Mauger, M Kernaleguen, C Lallemand… - …, 2018 - Taylor & Francis
… -specific DNA methylation technologies for potential DNA methylation-based biomarker …
methylation assays, but still failed to detect a considerable number of differences in the analyzed

Covalent genomic DNA modification patterns revealed by denaturing gradient gel blots

SL Laprise, MR Gray - Gene, 2007 - Elsevier
… to reveal differential methylation patterns between glioma tumor and leukocyte DNA samples
(Uhlmann et al., 1999). In this study, two to eleven methylation differences were revealed in …

High-speed conversion of cytosine to uracil in bisulfite genomic sequencing analysis of DNA methylation

M Shiraishi, H Hayatsu - DNA research, 2004 - academic.oup.com
… ing from the denaturation step. The increased speed of bisulfite genomic sequencing … 2000,
Large-scale methylation analysis of human genomic DNA reveals tissue-specific differences