Methylation of somatic vs germ cell DNAs analyzed by restriction endonuclease digestions
J Kaput, TW Sneider - Nucleic acids research, 1979 - academic.oup.com
… M in NaCl by adding solid NaCl, denatured at 75C for 30 minutes, and … different methylation
patterns 1n bovine DNA as compared to Novikoff rat hepatoma DNA and to the methylation …
patterns 1n bovine DNA as compared to Novikoff rat hepatoma DNA and to the methylation …
The methylation status of plant genomic DNA influences PCR efficiency
KV Kiselev, AS Dubrovina, AP Tyunin - Journal of plant physiology, 2015 - Elsevier
… methylated plant DNA regions. We suggest that increased DNA denaturation temperatures
of the highly methylated … cycles of amplification, since the difference in PCR product yield for …
of the highly methylated … cycles of amplification, since the difference in PCR product yield for …
Comparison of genome‐wide analysis techniques to DNA methylation analysis in human cancer
N Soozangar, MR Sadeghi, F Jeddi… - Journal of cellular …, 2018 - Wiley Online Library
… In this technique, following sonication, DNA fragments are denatured by heat treatment and
… This technique has also been used to analyze differences in CpG site methylation in DNA …
… This technique has also been used to analyze differences in CpG site methylation in DNA …
Differences in the binding of methylated albumin to non-replicating, replicating and denatured DNA from Ehrlich ascites cells
T Hofstaetter, H Probst - Biochimica et Biophysica Acta (BBA)-Nucleic …, 1978 - Elsevier
… ], and since denatured DNA is eluted from … methylated albumin to replicating DNA in the
test tube and compared the results with those obtained with fully double-stranded and denatured …
test tube and compared the results with those obtained with fully double-stranded and denatured …
Analysis of DNA methylation during germination of pepper (Capsicum annuum L.) seeds using methylation-sensitive amplification polymorphism (MSAP)
… , and significant differences in cytosine methylation levels have … Here, we report on the
analysis of cytosine methylation … and 0.01% w/v xylene cyanol), denatured at 95 C for 4 min and …
analysis of cytosine methylation … and 0.01% w/v xylene cyanol), denatured at 95 C for 4 min and …
A study of chemically methylated deoxyribonucleic acid
J Ramstein, C Hélène, M Leng - European Journal of …, 1971 - Wiley Online Library
… The difference between effects in vivo and in vitro has been ascribed to … methylated DNA
is not locally denatured. Stacking of methylated residues still occurs in denatured methylated …
is not locally denatured. Stacking of methylated residues still occurs in denatured methylated …
Genome-wide analysis of DNA methylation patterns
D Zilberman, S Henikoff - 2007 - journals.biologists.com
… DNA is denatured and then treated with sodium … methylated DNA. Although less of an issue
with McrBC, sequence polymorphisms between samples can mimic methylation differences if …
with McrBC, sequence polymorphisms between samples can mimic methylation differences if …
Enrichment of Methylated Molecules Using Enhanced-Ice-Co-Amplification at Lower Denaturation Temperature-PCR (E-Ice-COLD-PCR) for The Sensitive Detection …
F Mauger, M Kernaleguen, C Lallemand… - …, 2018 - Taylor & Francis
… -specific DNA methylation technologies for potential DNA methylation-based biomarker …
methylation assays, but still failed to detect a considerable number of differences in the analyzed …
methylation assays, but still failed to detect a considerable number of differences in the analyzed …
Covalent genomic DNA modification patterns revealed by denaturing gradient gel blots
SL Laprise, MR Gray - Gene, 2007 - Elsevier
… to reveal differential methylation patterns between glioma tumor and leukocyte DNA samples
(Uhlmann et al., 1999). In this study, two to eleven methylation differences were revealed in …
(Uhlmann et al., 1999). In this study, two to eleven methylation differences were revealed in …
High-speed conversion of cytosine to uracil in bisulfite genomic sequencing analysis of DNA methylation
M Shiraishi, H Hayatsu - DNA research, 2004 - academic.oup.com
… ing from the denaturation step. The increased speed of bisulfite genomic sequencing … 2000,
Large-scale methylation analysis of human genomic DNA reveals tissue-specific differences …
Large-scale methylation analysis of human genomic DNA reveals tissue-specific differences …