… All methods for gene-specific methylation analysis described in this review either use
MSREs or bisulfite. For a more comprehensive review of methods allowing identification of 5-…

… The aim of this review is to inform biologists studying DNA methylation of the pros and cons
… key factors that must be considered when choosing a method for DNA methylation analysis: …

… DNA methylation analysis, with a particular emphasis on genome-scale DNA methylation
analysis … challenges that are unique to DNA methylation analysis are outlined. Although some …

… This article will not address the mechanisms by which CpG methylation affects … field of DNA
methylation analysis. We will describe different approaches for analysis of DNA methylation, …

… the role of DNA methylation in both development and disease states [6, 7]. Detection of DNA
methylation is based on the ability to distinguish cytosine from 5mC in the DNA sequence. …

… technique that is capable of quantitating DNA methylation at a The analysis of DNA
methylation patterns at indiparticular locus by using DNA oligonucleotides that anneal differ- …

… quantitative analysis is very important for DNA methylation analysis. … methylation patterns
are found in many diseases, especially cancer, where an overall decrease in DNA methylation …

… methylation patterns, for example, the total level of 5-methyl cytosine in the genome or the
frequency of methylation … been an explosion of interest in DNA methylation, and with it, many …

… The methylation status of cytosines in CpGs and occasionally in non-CpG … –DNA interactions,
gene expression, and chromatin structure and stability. The degree of DNA methylation at …

… -wide DNA methylation analysis, we recommend two available review articles (74,75). Finally,
it is critical to keep in mind that the results of PCR-based DNA methylation methodologies …