… We show here that digital PCR can benefit both DNA methylation analysis strategies. Digital
… for purposes of identifying and counting individual DNA molecule sequence alterations, and …

… compared five analytical methods: (i) MethyLight qPCR, (ii) MethyLight digital PCR (dPCR), …
targeted in this study, 47% of nucleotides within the MethyLight p14 assay target region are …

… Our analyses demonstrated the capacity of MethyLight ddPCR to detect a single methylated
NTRK3 … As a proof of principle study for applying ddPCR to DNA methylation analysis and …

… the experimental steps of MethyLight analysis, we present here … for quantitative methylation
analysis of bisulfite converted DNA. … DNA methylation analysis of bisulfite converted DNA. …

… DNA methylation analysis. Among them, the … MethyLight assay for the detection of DNA
methylation biomarkers for cancer diagnosis. Digital PCR is based on the limit dilution of the DNA …

… low levels of methylated DNA and thus fully utilize DNA methylation as a field … assay for
detecting methylated DNA based on droplet digital PCR, called the MethyLight ddPCR assay, We …

… methylation analysis 41 . Digital MethyLight is shown to have greater sensitivity and …
Taking an approach similar to that used in this study, multiplex MethyLight assays can be further …

… first introduce principles of DNA methylation analysis, and then … In real-time MSP and
MethyLight, DNA methylation level can … Advantage of digital MethyLight is absolute quantification …

… known from qMSP/MethyLight analyses [17, 18] and was recently also emphasized by Uehiro
and colleagues for ddPCR DNA methylation analyses [13]. In MethyLight, the transposable …

… For example, the study initially validating digital MethyLight is a case where digital-BSP
was the best choice, since it allowed accurate validation of another single-molecule technique …