… The first application of melting curve analysis to the profiling of DNA methylation was … of
high-resolution melting analysis (HRM) (3). We applied HRM to methylation analysis in the …

… Background: Most PCR assays for detection of 5-methylcytosine in genomic DNA entail a
two-… Methods: An in-tube methylation assay is described that integrates amplification of bisulfite-…

… To initially establish McMSP and McCOBRA as reliable methylation assays, we first determined
the methylation status of … DNA methylation analysis: a review of current methodologies. …

… is that when an unambiguous melting profile is obtained, there … for DNA methylation profiling
by melting curve analyses published … For the majority of our assays, which were designed to …

… -specific DNA methylation analysis is that methylation marks are … The melting curves or
derived melting peaks provide a … Compared with current gel-based assays (6), melting analysis …

… and simplify methylation analyses by reducing starting DNA … allowing quantitative methylation
analyses targeting individual … PCR assays for the detection of aberrant DNA methylation …

… methylation at specific CpGs. We describe an objective assessment of overall methylation
using melt curve analysis, a … Worm applied this principle to DNA methylation analysis [9], and a …

… in DNA methylation patterns are common in most cancers, occur early in carcinogenesis and
can be detected in body fluids. Reliable and sensitive quantitative assays … Melting curves …

… showing promise is DNA methylation, an epigenetic feature that changes in response to
environmental exposure [2], [3], [4]. The principle behind DNA methylation analysis on samples …

… amplification products has also been used to study DNA methylation [8–10] but … The swabs
were collected in triplicate and assayed at 1 month intervals (up to … DNA methylation analysis …