… Our results indicate that off-target DNA methylation primarily occurs when the dCas9 … highest
level of DNA methylation are located within ~400 bp of the sgRNA’s protospacer, dCas9-MC…

… of targeted DNA methylation by CRISPR gRNA-guided dCas9 methyltransferases, we used
… the characteristics of dCas9 methyltransferase-mediated DNA methylation in human cells. …

… , dCas9 targeted DNA methylation was described by two other groups using dCas9 fused to
… Vojta and colleagues observed efficient targeted methylation occurring in a distance of ∼35 …

… /Cas9 (dCas9)-based targeted DNA methylation editing tools … and the role of aberrant
methylation in various diseases. … editing used direct fusion of the bacterial methyltransferase M…

… that dCas9 specifically inhibits DNA methylation of targeted … on-target DNA methylation with
dCas9 without affecting the … DNA span that is protected from methylation by bound dCas9, …

… methyltransferases, which use S-Adenosyl methionine as the methyl donor to catalyze the
… CRISPR/dCas9-based system (CRISPR/dCas9-R2) to reduce the DNA methylation level by …

… dCas9-methyltransferases in pluripotent cells. We observe widespread off-target activity of
dCas9-methyltransferases… of CGIs from the de novo DNA methylation machinery 23,24 , which …

… of epigenetic modifications, including DNA methylation, has been … DNA methylation consisting
of deactivated Cas9 (dCas9) nuclease and catalytic domain of the DNA methyltransferase …

… methylation. Here, in E. coli, we show that shortening the dCas9-MC linker increases methylation
of … We speculate that shortening the linker may increase DNA methylation at the most …

… DNA methyltransferase to achieve three different methylation … dCas9-VP160 or dCas9-TET1,
but, surprisingly, we detected decreased DNA methylation in cells transfected with dCas9-…