… and negative regulatory elements at the H19/Igf2 locus, we analyzed two mouse mutations.
… of both H19 and Igf2 in mesodermal tissues to sequences downstream of the H19 gene. …

… of the IGF2 DMR (P = 0.004). A preliminary analysis suggested an association between H19
DMR … Our study shows that variation in DNA methylation of the IGF2/H19 locus is mainly …

… As discussed in this review, the methylation of DMRs within the Igf2-H19 locus is erased in
pluripotent stem cells, such as PGCs [6–11] and VSELs [12, 13] (Figure 2, middle panel). This …

… Lack of an open reading frame infers that H19 functions as an RNA 31, 32, however, apart
from involvement of H19 locus in imprinted regulation of Igf2, the physiological role of H19 …

… Our functional analysis of the IGF2-H19 locus in therians shows that regulation by the H19
DMD is the most ancient complex imprinting mechanism identified so far. All other imprinting …

… The H19/Igf2 locus and the current postulated model for regulation of imprinted expression
of the two genes are shown in Fig. 1b. This is often described as the ‘boundary model’, …

Igf2 and H19 exhibit parent-of-origin-specific monoallelic expression. H19 is expressed from
the maternal chromosome and Igf2 … -acting sequences upstream of the H19 promoter. In this …

… in Wilms tumors with relaxed imprinting of IGF2, the changes in DNAmethylation-ie, the gain
of methylation atHl9 and the loss of methylation at IGF2-are confined to the IGF2/H19 locus. …

… Upon further analysis, only one clone (E2.2) maintained a 1:1 ratio of band intensities of
wild-type to mutant at the H19 locus. These cells were injected into C57B1/6J blastocysts {…

… H19 gene with a differentially methylated domain (DMD) that lies approximately 2–4 kb upstream
of H19 … In the remainder of tissues that express Igf2 and H19, the cis elements that drive …