Profiling the DNA methylation patterns of imprinted genes in abnormal semen samples by next-generation bisulfite sequencing

W He, Υ Sun, S Zhang, X Feng, M Xu, J Dai… - … and Genetics, 2020 - Springer
… Our investigation confirmed that the imprinted gene DNA methylation levels were related to
… than 60 imprinted gene DMRs by RRBS and found that the imprinted regions of genes such …

Methylation dynamics of imprinted genes in mouse germ cells

D Lucifero, C Mertineit, HJ Clarke, TH Bestor… - Genomics, 2002 - Elsevier
… used bisulfite sequencing to determine the methylation dynamics of the imprinted genes
small … gene 3 (Peg3), and H19 fetal liver mRNA (H19). We identified regions in the maternally …

Bisulfite sequencing and dinucleotide content analysis of 15 imprinted mouse differentially methylated regions (DMRs): paternally methylated DMRs contain less …

H Kobayashi, C Suda, T Abe, Y Kohara… - … and genome research, 2006 - karger.com
… Indeed, many imprinted genes have differentially methylated … Imprinted genes in mammals
show monoallelic expression … expression of nearby imprinted genes and the establishment of …

Bisulfite sequencing in preimplantation embryos: DNA methylation profile of the upstream region of the mouse imprintedH19Gene

PM Warnecke, JR Mann, M Frommer, SJ Clark - Genomics, 1998 - Elsevier
… in the initial stages of embryogenesis we have modified the bisulfite sequencing technique
to study the methylation profile of the H19 imprinted gene in one- and two-cell embryos, in …

Deep bisulfite sequencing of aberrantly methylated loci in a patient with multiple methylation defects

J Beygo, O Ammerpohl, D Gritzan, M Heitmann… - PLoS …, 2013 - journals.plos.org
imprinted genes among the 85 conspicuous genes in the patient, we selected 19 (mainly
hypomethylated) genes for deep bisulfite amplicon sequencing … proved to be imprinted in blood…

Using long-read sequencing to detect imprinted DNA methylation

S Gigante, Q Gouil, A Lucattini, A Keniry… - Nucleic acids …, 2019 - academic.oup.com
… with those from Reduced Representation Bisulfite Sequencing data and through comparison
… We found 135 genes with a parental bias in expression (imprinted genes, 10% FDR, Figure …

A novel approach identifies new differentially methylated regions (DMRs) associated with imprinted genes

S Choufani, JS Shapiro, M Susiarjo… - Genome …, 2011 - genome.cshlp.org
… that AXL is a candidate imprinted gene. To further validate the parent of origin-specific
methylation patterns in this genomic region, we performed bisulfite sequencing of 14 CpG sites …

… -wide DNA methylation analysis of patients with imprinting disorders identifies differentially methylated regions associated with novel candidate imprinted genes

LE Docherty, FI Rezwan, RL Poole, H Jagoe… - … of medical genetics, 2014 - jmg.bmj.com
… , more recently, genome-wide bisulfite sequencing has allowed direct assessment of allele-…
of imprinted genes is approaching completion, with few novel germline imprints remaining …

De novo quantitative bisulfite sequencing using the pyrosequencing technology

JM Dupont, J Tost, H Jammes, IG Gut - Analytical biochemistry, 2004 - Elsevier
… In effect, there is a loss of heterozygosity of these genes, which consequently behave as …
200 imprinted genes, of which perhaps 50 are currently known (see catalog of imprinted genes

[HTML][HTML] … of embryonic germ cells from post migratory primordial germ cells, and methylation analysis of their imprinted genes by bisulfite genomic sequencing

SW Shim, DW Han, JH Yang, BY Lee, SB Kim… - Molecules and cells, 2008 - Elsevier
… had been lost in all of nine imprinted genes except Igf2 and H19. Igf2 … the methylation patterns
of imprinted genes in EGCs do not … For this analysis, we employed the bisulfite sequencing