… Here we describe the first study of the methylation status of two imprinted genes, H19 and
MEST, in these sperm cells, obtained from testicular biopsies of patients with different causes …

… In this study, testicular sperm were isolated from testicular tissue for analysis of DNA
methylation at imprinted genes. Our results demonstrate a significant decrease in DNA methylation …

… cases with impaired sperm DNA integrity). We detected the methylation status of 257 CpG
sites among two imprinted genes (H19 and SNRPN) and four non-imprinted genes related to …

… use of testicular spermatozoa for ICSI raised the question whether the genetic imprinting of
… Therefore, in this study, we analysed the methylation state of the paternally imprinted gene …

… In the male germ line, methylation of imprinted genes … imprinting analysis in testicular sperm
cells do not indicate incomplete establishment of the paternal imprint in elongated testicular …

… marks that regulate monoallelic expression of imprinted genes. In males, this involves erasure
… /obesity and DNA methylation status of imprinted gene regulatory regions in the gametes. …

… Marques CJ, Francisco T, Sousa S, Carvalho F, Barros A & Sousa M 2010 Methylation
defects of imprinted genes in human testicular spermatozoa. Fertility and Sterility [in press]. DOI:…

… semen quality, biochemical and histological analysis, we chose 6 paternally expressed
imprinted genes … changes in semen quality, serum testosterone level and testicular morphology …

… The sperm chromatin of fertile men retains a small number of nucleosomes that are … gene
promoters and imprinted gene loci. This unique chromatin packaging at certain gene promoters …

… in cases with disrupted spermatogenesis might derive from different imprinting marks in
the testicular adult germinal stem cell pool, or from defects occurring during progenitor diploid …