… Either whole- or reduced-genome approaches have been used to get the most comprehensive
DNA methylation profiles in organisms of various genome sizes. Copyright © 2009 John …

… large-scale sequencing projects; however, generating libraries has become the bottleneck in
DNA methylation profiling … its use for large-scale DNA methylation profiling, we modified the …

… of DNA methylation provide an important level of genomic regulation. In this article, we review
methods for analyzing these genome-wide … using genome-wide DNA methylation profiling. …

… DNA methylation variation among healthy individuals. We performed bioinformatic analysis
of high-resolution methylation profiles … variation between DNA methylation profiles are used …

… methylation assays, along with comparisons and biological examples, to provide an overview
of DNA methylation profiling … technologies make genome-wide profiling possible at a single…

… to genome-wide scale using arrays and next… DNA methylation measurements. This protocol
should enable any standard molecular biology laboratory to generate RRBS libraries of high …

… This results in a reduction of sequence complexity, and consequently, it becomes difficult
to design enough unique probes to extend this approach to a genome-wide scale. However, …

… The majority of restriction enzymes used for methylation profiling are precise and … The
bisulfite approach, however, has been difficult to apply on a genome-wide scale for mammals for …

… for its ability to quantify the DNA methylation status of essentially all … -scale DNA methylation
analysis 3,4 . Here we show that RRBS is highly appropriate for DNA methylation profiling of …

… genome-scale DNA methylation profiles at nucleotide resolution in mammalian cells. Using
high-… It has been impractical to apply bisulphite sequencing at a genome-wide scale because …