… We have developed a series of plasmid vectors and host strains for conditional … ” plasmid
vectors and host strains for conditional overexpression of halophilic proteins in H. volcanii. The …

… cloning sites in these vectors and the lack of a direct selection for plasmids with cloned se…
new shuttle vectors with numerous unique cloning sites. One of these plasmids, pMLH3, …

… vectors are now routinely used to investigate the molecular biology of these organisms. …
Haloferax volcanii-Escherichia coli shuttle vector pWL102 (11), based on the Haloferax volcanii …

… Here we report the optimization of two steps of generating plasmids for deletion mutant
construction. The optimized cloning method is faster and more efficient than the previously …

… The development of plasmid-based genetic exchange systems in the archaebacteria has …
Haloferax volcanii (formerly Halobacterium volcanii), has made it possible to develop plasmids …

… Haloferax volcanii is an obligate halophilic archaeon with its origin in the Dead Sea. Simple
… This plasmid has the potential to make biotechnological processes cost effective since its …

… Haloferax volcanii has a multireplicon genome, consisting of a main chromosome, three
secondary chromosomes, and a plasmid. … found on all replicons except plasmid pHV2. However, …

… Haloferax volcanii is particularly suited for genetic analysis in … and there are several plasmid
vectors available. There are a … However, there are currently no vectors that allow controlled …

… the replication of certain plasmids, eg … Haloferax chromosome and introduced vector
plasmids, we sought a selectable marker that showed less sequence similarity to the Hfx. volcanii …

… The pleomorphic archaeon Haloferax volcanii commonly forms discoid-shaped (‘plate’) cells
… Improved strains and plasmid vectors for conditional overexpression of His-tagged proteins …