… The problems and advantages of the different methods for detecting aberrant methylation
are discussed. As the number of genes known to be hypermethylated in cancer is growing, the …

… In the present study, we have isolated regions of DNA that showed methylation differences
between normal and tumor colon tissue and cell lines. Using various primer combinations …

… Gonzalgo and Jones (7) modified this method for the quantification of DNA methylation
differences at specific CpG sites. In contrast with COBRA, this method requires radioisotope …

… investigate differences between these two techniques in the assessment of DNA methylation
and … Although there is substantial accordance in the results obtained using these different …

… arbitrarily primed methylation sensitive polymerase chain reaction (PCR… DNA methylation
profiles in different cell types. Like other DNA fingerprinting techniques, such as RAPD or DNA …

… We compare three different approaches to identify mixed MSP amplification products of the
GSTP1 gene from prostate cancer patient samples. The simplest method involves restriction …

… DNA methylation (23,24). Subsequent amplification of bisulfite-converted DNA by polymerase
chain reaction (… dependent on the methylation status and how the two different MSP primer …

… compare carcinoembryonic antigen (CEA)-specific reverse transcriptase-polymerase chain
reaction (RT-PCR) and methylation-… out to analyze methylation status of three different genes …

… pyrosequencing, we identified significant DNA methylation differences at the ID4 gene …
Methylation-specific polymerase chain reaction Methylation-specific polymerase chain reaction …

… polymerase chain reaction (PCR)] being widely used to provide single-base quantitative
measurement of cytosine methyl-modifications (5mC and 5-hydroxymethylcytosine, 5hmC). The …