Comparison of methyl-DNA immunoprecipitation (MeDIP) and methyl-CpG binding domain (MBD) protein capture for genome-wide DNA methylation analysis reveal …
… methylated DNA regions of the genome, namely methylDNA … capture of methylated DNA
using a methyl-CpG binding domain-based (MBD) protein to discover differentially methylated …
using a methyl-CpG binding domain-based (MBD) protein to discover differentially methylated …
A novel method to capture methylated human DNA from stool: implications for colorectal cancer screening
H Zou, J Harrington, RL Rego, DA Ahlquist - Clinical chemistry, 2007 - academic.oup.com
… method to capture methylated human DNA from stool samples using MBD protein chelated
… Of note, our results show that MBD protein has very low affinity to bacterial DNA, which has …
… Of note, our results show that MBD protein has very low affinity to bacterial DNA, which has …
A SILAC-based screen for Methyl-CpG binding proteins identifies RBP-J as a DNA methylation and sequence-specific binding protein
SJJ Bartels, CG Spruijt, AB Brinkman, PWTC Jansen… - PloS one, 2011 - journals.plos.org
… , three BTB/POZ-Zn-finger proteins, and UHRF1. Most of the … other proteins are capable of
recognizing methylated DNA. … , proteins are captured from a protein extract by DNA coupled …
recognizing methylated DNA. … , proteins are captured from a protein extract by DNA coupled …
DNA methylation methods: Global DNA methylation and methylomic analyses
S Li, TO Tollefsbol - Methods, 2021 - Elsevier
… DNA methylation generally refers to the covalent addition of methyl groups to the 5-carbon
of … In the process of an active DNA demethylation, TET proteins can oxidize 5mC to 5hmC, 5-…
of … In the process of an active DNA demethylation, TET proteins can oxidize 5mC to 5hmC, 5-…
Development of a method to measure DNA methylation levels by using methyl CpG-binding protein and luciferase-fused zinc finger protein
D Hiraoka, W Yoshida, K Abe, H Wakeda… - Analytical …, 2012 - ACS Publications
… To capture methylated DNA, 200 μL of the DNA with various concentrations in binding … the
beads, and captured DNA was eluted by heating at 95 C for 20 min. The eluted DNA was used …
beads, and captured DNA was eluted by heating at 95 C for 20 min. The eluted DNA was used …
Methyl-binding DNA capture sequencing for patient tissues
RR Jadhav, YV Wang, YT Hsu, J Liu, D Garcia… - JoVE (Journal of …, 2016 - jove.com
… of these techniques called Methyl-Binding DNA Capture sequencing (MBDCap… methylated
regions of the DNA. This technique builds upon the methyl-binding ability of the MBD2 protein …
regions of the DNA. This technique builds upon the methyl-binding ability of the MBD2 protein …
Enrichment of methylated DNA by methyl-CpG immunoprecipitation
M Sonnet, C Baer, M Rehli, D Weichenhan… - Lymphoma: Methods and …, 2013 - Springer
… fusion protein, … protein is immobilized to protein A-coated sepharose or magnetic bead
particles. Upon incubation with fragmented sample DNA, MBD2 selectively captures methylated …
particles. Upon incubation with fragmented sample DNA, MBD2 selectively captures methylated …
A novel methyl-binding domain protein enrichment method for identifying genome-wide tissue-specific DNA methylation from nanogram DNA samples
… /MBD3L1 enrichment for methylated DNA, kinase pre-… DNA. We have shown that MeKL-chip
can be applied to as little as 20 ng of DNA, enabling comprehensive analysis of small DNA …
can be applied to as little as 20 ng of DNA, enabling comprehensive analysis of small DNA …
Methyl-DNA immunoprecipitation (MeDIP): hunting down the DNA methylome
FV Jacinto, E Ballestar, M Esteller - Biotechniques, 2008 - Future Science
… , several strategies involving the specific interaction of proteins with methylated DNA have …
family of proteins, which are chosen for their selectivity in binding methylated DNA. Strategies …
family of proteins, which are chosen for their selectivity in binding methylated DNA. Strategies …
Direct DNA methylation profiling using methyl binding domain proteins
Y Yu, S Blair, D Gillespie, R Jensen, D Myszka… - Analytical …, 2010 - ACS Publications
… Capture probe oligonucleotides are singly methylated at all CpG … proteins with methylated
DNA, we chose CpG-rich target oligonucleotide sequences from the O6-methylguanine DNA …
DNA, we chose CpG-rich target oligonucleotide sequences from the O6-methylguanine DNA …
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