… can be targeted for cross-linking including the N7 of guanine, the C-5 methyl … capture-mass
spectrometry experiments have identified a wide variety of proteins forming DNA–protein …

… proteins can be used for the study of interaction with methylated DNA and the assays for DNA
… had a histidine-tag at their C-terminal, AC was chosen as the first capture step. Each crude …

… Using the MMR protein MLH1 as bait, we cloned MED1. The MED1 protein forms a complex
with MLH1, binds to methyl-CpG-containing DNA, has homology to bacterial DNA repair …

… of bound proteins or protein complexes on DNA was … hemi-methylated DNA with a mismatch,
we find that looped DNA … site; 17 of 47 traced, looped DNA appeared to bridge the sites (10 …

… Alkylation of DNA by endogenous methyl donors, … the DNA backbone to create an optimized
protein–DNA binding surface. In both 3d3mA•T and G•T complexes, specific protein–DNA …

… , including methyl-CpG binding domain (MBD) proteins which recruit the enzymatic … function
of DNA methylation, DNA methyltransferases, and methyl-CpG binding proteins in vertebrate …

… To capture methylated DNA, 200 μL of the DNA solution at various concentrations in … the
beads, and captured DNA was eluted by heating at 95 C for 20 min. The eluted DNA was used …

… unit, has been applied for combining more biotinylated capture probe DNA S1 in this work. …
recognition between oligonucleotide sequence, DNA MTase and methyl CpG binding protein. …

… Formaldehyde is also used under similar experimental conditions in chromatin
immunoprecipitation (ChIP) experiments as the cross-linking agent that captures protein-DNA …

… candidate methylated proteins, and it recognizes a wider range of methylated proteins than
… In an experiment analyzing proteins captured by 3xMBT from yeast lysate, the most strongly …