Purification of specific cell population by fluorescence activated cell sorting (FACS)

S Basu, HM Campbell, BN Dittel, A Ray - JoVE (Journal of Visualized …, 2010 - jove.com
… studies often require highly purified cell populations. FACS is a technique of choice to purify
cell populations of known phenotype. Other bulk methods of purification include panning, …

[HTML][HTML] … clustering and lineage markers: insights into cellular differentiation and gene function from large-scale microarray studies of purified primary cell populations

DA Hume, KM Summers, S Raza, JK Baillie… - Genomics, 2010 - Elsevier
… generated from 44 purified primary mouse cell populations or untransformed cells and two
mouse … mRNA from various leukocyte populations, generally purified by FACS using lineage-…

Efficient detection and purification of cell populations using synthetic microRNA switches

K Miki, K Endo, S Takahashi, S Funakoshi, I Takei… - Cell stem cell, 2015 - cell.com
… the cells of interest. However, specific antigens for many cell types have not been identified,
making their isolation difficult. Here, we describe an efficient method for purifying cells based …

Purification and characterization of heterogeneous pluripotent hematopoietic stem cell populations expressing high levels of c-kit receptor

D Orlic, R Fischer, S Nishikawa, AW Nienhuis… - 1993 - ashpublications.org
purified, and highly purified bone marrow populationspopulations of Lin- c-kitDULL and
Linc-kitBR cells was pooled for each assay. Five micrograms of total RNA from each population

Differential DNA methylation analysis optimally requires purified cell populations

MK Skinner - Fertility and sterility, 2016 - fertstert.org
… a mixed cell population analysis needs to be noted and confirmed with purified cell populations.
… resources may facilitate mixed cell population studies and not require confirmation with …

Demystifying SP cell purification: viability, yield, and phenotype are defined by isolation parameters

F Montanaro, K Liadaki, J Schienda, A Flint… - Experimental cell …, 2004 - Elsevier
… , tissue of origin, and relationships among SP cell populations extracted from different
tissues. Studies on bone marrow SP cells have followed a consistent protocol for their isolation …

Isolation of a purified epithelial cell population from human colon

JK Roche - Colorectal Cancer: Methods and Protocols, 2001 - Springer
While in situ techniques have been valuable in identifying the presence and localization of
cytoplasmic and membrane components in tissue ( 1 ), there is often a need to study directly …

Cell purification: a new challenge for biobanks

M Almeida, AC García-Montero, A Orfao - Pathobiology, 2015 - karger.com
… and highquality purified cell populations for the … purified cell populations or their biological
components (eg DNA, RNA, proteins). Therefore, a clear opportunity exists for preparative cell

Purification, amplification and characterization of a population of human erythroid progenitors

Freyssinier, Amsellem, Picard, Ducrocq… - British journal of …, 1999 - Wiley Online Library
… of erythroid cell populations of rather mature stages. Other purification techniques requiring
purification and expansion of a homogenous population of immature erythroid progenitors. …

[HTML][HTML] Efficient ex vivo engineering and expansion of highly purified human hematopoietic stem and progenitor cell populations for gene therapy

E Zonari, G Desantis, C Petrillo, FE Boccalatte… - Stem cell reports, 2017 - cell.com
… the CD38 dim populations, we could interrogate the entire CD34 + cell population for
differential … One day after transduction, we re-assembled the following cell populations for …