Detection of DNA methylation in the genome has been possible for decades; however, the
ability to deliberately and specifically manipulate local DNA methylation states in the …

Mammalian genomes exhibit complex patterns of gene expression regulated, in part, by
DNA methylation. The advent of engineered DNA methyltransferases (MTases) to target …

Tools for actively targeted DNA demethylation are required to increase our knowledge about
regulation and specific functions of this important epigenetic modification. DNA …

DNA methylation is the most widely-studied epigenetic modification, playing a critical role in
the regulation of gene expression. Dysregulation of DNA methylation is implicated in the …

DNA methylation plays a critical role in the regulation and maintenance of cell-type specific
transcriptional programs. Targeted epigenome editing is an emerging technology to …

Here we develop a methylation editing toolbox, Casilio-ME, that enables not only RNA-
guided methylcytosine editing by targeting TET1 to genomic sites, but also by co-delivering …

Mammalian DNA methylation is a critical epigenetic mechanism orchestrating gene
expression networks in many biological processes. However, investigation of the functions …

Comprehensive studies have shown that DNA methylation plays vital roles in both loss of
pluripotency and governance of the transcriptome during embryogenesis and subsequent …

DNA methylation is important for establishing and maintaining cell identity and for genomic
stability. This is achieved by regulating the accessibility of regulatory and transcriptional …

DNA methylation contributes to the maintenance of genomic integrity in somatic cells, in part
through the silencing of transposable elements. In this study, we use CRISPR-Cas9 …