Targeted DNA demethylation of the Fgf21 promoter by CRISPR/dCas9-mediated epigenome editing
N Hanzawa, K Hashimoto, X Yuan, K Kawahori… - Scientific reports, 2020 - nature.com
Recently, we reported PPARα-dependent DNA demethylation of the Fgf21 promoter in the
postnatal mouse liver, where reduced DNA methylation is associated with enhanced gene …
postnatal mouse liver, where reduced DNA methylation is associated with enhanced gene …
Large-scale manipulation of promoter DNA methylation reveals context-specific transcriptional responses and stability
Background Cytosine DNA methylation is widely described as a transcriptional repressive
mark with the capacity to silence promoters. Epigenome engineering techniques enable …
mark with the capacity to silence promoters. Epigenome engineering techniques enable …
[PDF][PDF] DNA methylation regulates alternative polyadenylation via CTCF and the cohesin complex
Dysregulation of DNA methylation and mRNA alternative cleavage and polyadenylation
(APA) are both prevalent in cancer and have been studied as independent processes. We …
(APA) are both prevalent in cancer and have been studied as independent processes. We …
Engineering of effector domains for targeted DNA methylation with reduced off-target effects
D Hofacker, J Broche, L Laistner, S Adam… - International journal of …, 2020 - mdpi.com
Epigenome editing is a promising technology, potentially allowing the stable reprogramming
of gene expression profiles without alteration of the DNA sequence. Targeted DNA …
of gene expression profiles without alteration of the DNA sequence. Targeted DNA …
Endonuclease-assisted PAM-free recombinase polymerase amplification coupling with CRISPR/Cas12a (E-PfRPA/Cas) for sensitive detection of DNA methylation
S Zhou, J Dong, L Deng, G Wang, M Yang, Y Wang… - ACS …, 2022 - ACS Publications
DNA methylation is considered as a potential cancer biomarker. The evaluation of DNA
methylation level will contribute to the prognosis and diagnosis of cancer. Herein, we …
methylation level will contribute to the prognosis and diagnosis of cancer. Herein, we …
Programmable RNA N6-methyladenosine editing by CRISPR-Cas9 conjugates
RNA modification in the form of N 6-methyladenosine (m6A) regulates nearly all the post-
transcriptional processes. The asymmetric m6A deposition suggests that regional …
transcriptional processes. The asymmetric m6A deposition suggests that regional …
Epitranscriptomic editing of the RNA N6-methyladenosine modification by dCasRx conjugated methyltransferase and demethylase
Abstract N6-methyladenosine (m6A) is a common modification on endogenous RNA
transcripts in mammalian cells. Technologies to precisely modify the RNA m6A levels at …
transcripts in mammalian cells. Technologies to precisely modify the RNA m6A levels at …
Dual methylation-sensitive restriction endonucleases coupling with an RPA-assisted CRISPR/Cas13a system (DESCS) for highly sensitive analysis of DNA …
X Wang, S Zhou, C Chu, M Yang, D Huo, C Hou - ACS sensors, 2021 - ACS Publications
High-performance detection of DNA methylation possesses great significance for the
diagnosis and therapy of cancer. Herein, for the first time, we present a digestion strategy …
diagnosis and therapy of cancer. Herein, for the first time, we present a digestion strategy …
Programmable RNA N1‐Methyladenosine Demethylation by a Cas13d‐Directed Demethylase
Abstract N1‐methyladenosine (m1A) is a prevalent and reversible RNA modification, which
plays a crucial role in the regulation of RNA fate and gene expression. However, the lack of …
plays a crucial role in the regulation of RNA fate and gene expression. However, the lack of …
meCLICK-Seq, a substrate-hijacking and RNA degradation strategy for the study of RNA methylation
The fates of RNA species in a cell are controlled by ribonucleases, which degrade them by
exploiting the universal structural 2′-OH group. This phenomenon plays a key role in …
exploiting the universal structural 2′-OH group. This phenomenon plays a key role in …