In most higher organisms, DNA is modified after synthesis by the enzymatic conversion of
many cytosine residues to 5-methylcytosine. For several years, control of gene activity by …

During the past few decades we have witnessed an era of remarkable growth in the field of
molecular biology. In 1950 very little was known of the chemical constitution of biological …

Cytosine methylation is a com-mon DNA modification found in most eukaryotic organisms
including plants, animals, and fungi (1, 2). The addition of a methyl group to cytosine …

Abstract Frog virus 3 (FV3) DNA is the most highly methylated DNA of any known DNA virus;
about 20% of the cytosine residues in FV3 DNA are methylated (D. Willis and A. Granoff …

ABOUT 3070 of cytosine residues in mammalian DNA are present as 5-methylcytosine,
methylation of cytosine usually occurring soon after DNA synthesis. The existence of such …

The methylated bases of DNA are formed by the transfer of the methyl group from S-
adenosylmethionine to a polynucleotide acceptor. This transfer is catalyzed by highly …

ARE changes in DNA methylation patterns a cause or an effect of eukaryotic gene
expression? There are already many examples of an inverse correlation between the level …

Epigenetic effects have often enough been viewed as inactivation of a gene translocated to
a position near verging on the mystical. It is a paradox of conventional heterochromatin …

Hemimethylated DNA substrates prepared from cell cultures treated with 5-azacytidine are
efficient acceptors of methyl groups from S-adenosylmethionine in the presence of a crude …

We describe a method that permits the study of the state of cytosine methylation and of in
vivo protein-DNA interactions in higher eukaryotes. This powerful technique is applicable to …