Apparent alterations in DNA methylation have been observed in many cancers, but whether
such alterations represent a persistent alteration in the normal methylation process is not …

It has long been held that reversible promoter methylation allows genes to be expressed in
the appropriate cell types during development. However, no endogenous gene has been …

The aim of pharmacology has traditionally been to develop compounds that could mimic,
activate or antagonize the molecules that control and regulate the physiological state of an …

DNA methylation has come of age. For more than 40 years, it has been known that
eukaryotes tag their DNA by the covalent addition of a methyl group to cytosine residues; …

The relationship between levels of DNA methylation and gene activity has been known for
some time. Many of the early procedures developed gave only somewhat limited information …

In this report we test the hypothesis that a cis-acting methylation center can induce
epigenetic gene inactivation. The cis-acting element used is an 838-base pair fragment that …

Hemimethylated duplex DNA of the bacteriophage ØX 174 was synthesized using primed
repair synthesis in vitro with E. coli DNA polymerase I followed by ligation to produce the …

Several lines of evidence strongly indicate that methylation of cytosine residues within CpG
dinucleotides are implicated in the regulation of gene expression in eukaryotes. Transfection …

Transformed cells can spontaneously silence genes by de novo methylation, and it is
generally assumed that this is due to DNA methyltransferase activity. We have tested the …

About 60% of CpG dinucleotides in vertebrate DNA are methylated at position 5 of the
cytosine base. DNA methylation is essential for mammalian development [11, but places a …