It is estimated that∼ 2500 genes are essential for the normal development of a zebrafish
embryo. A mutation in any one of these genes can result in a visible developmental defect …

To rapidly identify genes required for early vertebrate development, we are carrying out a
large-scale, insertional mutagenesis screen in zebrafish, using mouse retroviral vectors as …

Recently our laboratory described an efficient method for generating retroviral provirus
insertions in the zebrafish germ line, and we showed that provirus insertions induce …

An important technology in model organisms is the ability to make transgenic animals. In the
past, transgenic technology in zebrafish has been limited by the relatively low efficiency with …

As the first draft of the human genome nears completion, we are faced with the challenge of
defining the functions of the 70,000–100,000 genes contained within the sequence. A …

Using a combination of techniques we developed, we infected zebrafish embryos using
pseudotyped retroviruses and mapped the genomic locations of the proviral integrations in …

Publisher Summary Three approaches have been used successfully to clone genes defined
by mutations in zebrafish: retroviral tagging, testing candidate genes, and positional cloning …

With the goal of developing techniques for DNA insertional mutagenesis in zebrafish, we
established procedures for rapidly obtaining and injecting large numbers of fertilized eggs …

We studied the efficiency with which two chemical mutagens, ethyl methanesulfonate (EMS)
and N-ethyl-N-nitrosourea (ENU) can induce mutations at different stages of …

Exquisite embryonic lethal mutations have been isolated in hundreds of genes necessary for
zebrafish development. Analysis of this resource promises to enhance our understanding of …