Activity of a 2.5 S mouse myeloma DNA polymerase (termed DNA polymerase II) measured
with either poly (rA) or poly (dA) as template did not require sulfhydryl-reducing reagents, but …

The combination in reaction mixtures of 1 mM dithiothreitol and 1 mM p-
hydroxymercuribenzoate resulted in a 10-fold stimulation in the activity of a 2.5 S mouse …

Five distinct forms of DNA polymerase (deoxynucleosidetriphosphate: DNA
deoxynucleotidyltransferase, EC 2.7. 7.7) were separated from extracts of mouse myeloma …

Cytoplasmic (high‐molecular‐weight) DNA polymerase was partially purified from mouse
myeloma. Upon chromatography on DEAE‐Sephadex, following fractionation on …

Eighty four precent of the total extractable DNA polymerase a activity was associated with
nuclei isolated from mouse myeloma MOPC 104E cells in the presence of Ca2±. From the …

Unlike other rβ-class eukaryotic DNA polymerases, the enzyme purified from the Novikoff
hepatoma is inhibited by both sulfhydryl blocking agents N-ethylmaleimide (NEM) and p …

DNA polymerase [EC 2.7. 7.7] activities present in hypotonic extract from rat ascites
hepatoma AH130 cells were eluted in three separable peaks on DEAE-cellulose column …

A high molecular weight membrane-bound DNA polymerase from the mouse myeloma,
MOPC-104E, has been purified extensively, and characterizedwith re-gard to physical and …

Polyacrylamide gel electrophoresis should be useful for monitoring DNA polymerase (
deoxynucleosidetriphosphate: DNA deoxynucleotidyltransferase, EC 2.7. 7.7) preparations …

MATERIALS AND METHODS Chemicals-[Me-3H] Thymidine-5• Œ-triphos phate([3H] dTTP)
and other labeled compounds were purchased from New England Nuclear Co., USA …