The de novo methylation activity of Dnmt3a is distinctly different than that of Dnmt1

CL Hsieh - BMC biochemistry, 2005 - Springer
Abstract Background Though Dnmt1 is considered the primary maintenance
methyltransferase and Dnmt3a and Dnmt3b are considered de novo methyltransferases in …

Reconstitution and mechanism of the stimulation of de novo methylation by human DNMT3L

MS Kareta, ZM Botello, JJ Ennis, C Chou… - Journal of Biological …, 2006 - ASBMB
The DNMT3-like protein, DNMT3L, is required for germ line DNA methylation, although it is
inactive as a DNA methyltransferase per se. Previous studies have shown that DNMT3L …

DNMT3L stimulates the DNA methylation activity of Dnmt3a and Dnmt3b through a direct interaction

I Suetake, F Shinozaki, J Miyagawa… - Journal of Biological …, 2004 - ASBMB
In mammals, the resetting of DNA methylation patterns in early embryos and germ cells is
crucial for development. Two DNA methyltransferases, Dnmt3a and Dnmt3b, are …

Interactions within the mammalian DNA methyltransferase family

JB Margot, AE Ehrenhofer-Murray, H Leonhardt - BMC molecular biology, 2003 - Springer
Background In mammals, epigenetic information is established and maintained via the
postreplicative methylation of cytosine residues by the DNA methyltransferases Dnmt1 …

Preferential methylation of unmethylated DNA by mammalian de novo DNA methyltransferase Dnmt3a

T Yokochi, KD Robertson - Journal of Biological Chemistry, 2002 - ASBMB
DNA methylation is an epigenetic modification of DNA. There are currently three catalytically
active mammalian DNA methyltransferases, DNMT1,-3a, and-3b. DNMT1 has been shown …

A single amino acid substitution confers enhanced methylation activity of mammalian Dnmt3b on chromatin DNA

L Shen, G Gao, Y Zhang, H Zhang, Z Ye… - Nucleic acids …, 2010 - academic.oup.com
Dnmt3a and Dnmt3b are paralogous enzymes responsible for de novo DNA methylation but
with distinguished biological functions. In mice, disruption of Dnmt3b but not Dnmt3a causes …

Identification of DNMT1 (DNA methyltransferase 1) hypomorphs in somatic knockouts suggests an essential role for DNMT1 in cell survival

G Egger, S Jeong, SG Escobar… - Proceedings of the …, 2006 - National Acad Sciences
Previous studies have shown that DNA methyltransferase (Dnmt) 1 is required for
maintenance of bulk DNA methylation and is essential for mouse development. However …

Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA

M Fatemi, A Hermann, H Gowher… - European journal of …, 2002 - Wiley Online Library
Dnmt3a is a de novo DNA methyltransferase that modifies unmethylated DNA. In contrast
Dnmt1 shows high preference for hemimethylated DNA. However, Dnmt1 can be activated …

Enzymatic properties of recombinant Dnmt3a DNA methyltransferase from mouse: the enzyme modifies DNA in a non-processive manner and also methylates non …

H Gowher, A Jeltsch - Journal of molecular biology, 2001 - Elsevier
We present the first in vitro study investigating the catalytic properties of a mammalian de
novo DNA methyltransferase. Dnmt3a from mouse was cloned and expressed in …

Domain structure of the Dnmt1, Dnmt3a, and Dnmt3b DNA methyltransferases

S Tajima, I Suetake, K Takeshita, A Nakagawa… - … -Role and Function, 2022 - Springer
In mammals, three major DNA methyltransferases, Dnmt1, Dnmt3a, and Dnmt3b, have been
identified. Dnmt3a and Dnmt3b are responsible for establishing DNA methylation patterns …