In the last two decades, zebrafish has become one of the fastest growing model organisms
in terms of publications, however it has been plagued with the absence of a key tool in the …

Zebrafish is a model vertebrate suitable for genetic analysis. Forward genetic analysis via
chemical mutagenesis screening has established a variety of zebrafish mutants that are …

The rapid growth of the field of gene editing can largely be attributed to the discovery and
optimization of designer endonucleases. These include zinc finger nucleases (ZFNs) …

Zebrafish (Danio rerio) is a unique model organism at the functional intersection between a
high fecundity and conserved vertebrate physiology while being amenable to a multitude of …

Genome editing appears poised to enter an exciting new era. Targeted double-stranded
breaks due to custom restriction enzymes are powerful nucleating events for the induction of …

Over the last few years, the technology to create targeted knockout and knockin zebrafish
animals has exploded. We have gained the ability to create targeted knockouts through the …

Transcription activator-like effectors (TALEs) are important genomic tools with customizable
DNA-binding motifs for locus-specific modifications. In particular, TALE nucleases or …

The targeted introduction of mutations utilizing sequence specific transcription activator-like
effector nucleases (TALENs) and the clustered regularly interspaced short palindromic …

The use of zebrafish in functional genomics and disease modeling has become popular due
to the ease of targeted mutagenesis with genome editing nucleases, ie, zinc finger …

Zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs)
provide powerful platforms for genome editing in plants and animals. Typically, a single …