Assessment of DNA methylation has become a critical factor for the identification,
development and application of methylation based biomarkers. Here we describe a …

Background DNA methylation is an important epigenetic mechanism in several human
diseases, most notably cancer. The quantitative analysis of DNA methylation patterns has …

“Epigenetics” refers to a heritable change in transcriptional status without alteration in the
primary nucleotide sequence. Epigenetics provides an extra layer of transcriptional control …

Background DNA methylation biomarkers for early detection, risk stratification and treatment
response in cancer have been of great interest over the past decades. Nevertheless, clinical …

DNA methylation was the first epigenetic mark to be discovered, involving the addition of a
methyl group to the 5′ position of cytosine by DNA methyltransferases, and can be …

DNA methylation based biomarkers have considerable potential for molecular diagnostics,
both as tumor specific biomarkers for the early detection or post-therapeutic monitoring of …

Deregulation of the epigenome is now recognized as a major mechanism involved in the
development and progression of human diseases such as cancer. As opposed to the …

Here, we present a practical overview of four commonly used validation methods for DNA
methylation assessment: methylation specific restriction endonucleases (MSRE) analysis …

DNA methylation plays key roles in diverse biological processes such as X chromosome
inactivation, transposable element repression, genomic imprinting, and tissue-specific gene …

Epigenetic silencing by promoter methylation of genes associated with cancer initiation and
progression is a hallmark of tumour cells. As a consequence, testing for DNA methylation …