We describe here the isolation and characterization of a B-type DNA polymerase (PolB) from
the archaeonMethanobacterium thermoautotrophicum ΔH. Uniquely, the catalytic domains …

Since their initial characterization over 30 years ago, it has been believed that the archaeal
B-family DNA polymerases are single-subunit enzymes. This contrasts with the multi-subunit …

A mutant of the high fidelity family-B DNA polymerase from the archaeon Thermococcus
gorgonarius (Tgo-Pol), able to replicate past DNA lesions, is described. Gain of function …

The gene encoding the thermostable DNA polymerase from the archaeon Sulfolobus
solfataricus (strain MT 4) was isolated by means of two degenerate oligonucleotide probes …

One of the major evolutionary transitions that led to DNA replacing RNA as the primary
informational molecule in biological systems is still the subject of an intense debate in the …

Background Evolution of DNA polymerases, the key enzymes of DNA replication and repair,
is central to any reconstruction of the history of cellular life. However, the details of the …

Family D DNA polymerase has recently been found in the Euryarchaeota subdomain of
Archaea. Its genes are adjacent to several other genes related to DNA replication, repair …

Cenarchaeum symbiosum, an archaeon which lives in specific association with a marine
sponge, belongs to a recently recognized nonthermophilic crenarchaeotal group that …

Archaeal family B DNA polymerases contain a specialised pocket that binds tightly to
template-strand uracil, causing the stalling of DNA replication. The mechanism of this unique …

The interaction of archaeal family B DNA polymerases with deaminated bases has been
examined. As determined previously by our group, the polymerase binds tightly to uracil (the …